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Western Blot (WB) (WB Suggested Anti-DDX31 Antibody Titration: 2.5ug/mlELISA Titer: 1:1562500Positive Control: Jurkat cell lysate)

Rabbit DDX31 Polyclonal Antibody | anti-DDX31 antibody

DDX31 antibody - N-terminal region

Gene Names
DDX31; PPP1R25
Reactivity
Guinea Pig, Human, Mouse, Rat
Applications
Western Blot
Purity
Protein A purified
Synonyms
DDX31; Polyclonal Antibody; DDX31 antibody - N-terminal region; anti-DDX31 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Guinea Pig, Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Protein A purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sequence
Synthetic peptide located within the following region: QASSEAPPAKRRNETSFLPAKKTSVKETQRTFKGNAQKMFSPKKHSVSTS
Sequence Length
585
Applicable Applications for anti-DDX31 antibody
Western Blot (WB)
Homology
Guinea Pig: 83%; Human: 100%; Mouse: 86%; Rat: 93%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human DDX31
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(WB Suggested Anti-DDX31 Antibody Titration: 2.5ug/mlELISA Titer: 1:1562500Positive Control: Jurkat cell lysate)

Western Blot (WB) (WB Suggested Anti-DDX31 Antibody Titration: 2.5ug/mlELISA Titer: 1:1562500Positive Control: Jurkat cell lysate)
Related Product Information for anti-DDX31 antibody
This is a rabbit polyclonal antibody against DDX31. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX31 is a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants.
Product Categories/Family for anti-DDX31 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
64kDa
NCBI Official Full Name
probable ATP-dependent RNA helicase DDX31 isoform 2
NCBI Official Synonym Full Names
DEAD-box helicase 31
NCBI Official Symbol
DDX31
NCBI Official Synonym Symbols
PPP1R25
NCBI Protein Information
probable ATP-dependent RNA helicase DDX31
UniProt Protein Name
Probable ATP-dependent RNA helicase DDX31
UniProt Gene Name
DDX31

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2016]

Uniprot Description

DDX31: Probable ATP-dependent RNA helicase. Belongs to the DEAD box helicase family. DDX31/DBP7 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.13; Hydrolase; Nucleolus

Chromosomal Location of Human Ortholog: 9q34.13

Cellular Component: cytoplasm; Golgi apparatus; intracellular membrane-bound organelle; nucleolus

Molecular Function: ATP binding; ATP-dependent RNA helicase activity; protein binding; RNA binding

Biological Process: ribosome biogenesis; RNA secondary structure unwinding

Research Articles on DDX31

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Product Notes

The DDX31 ddx31 (Catalog #AAA3203160) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DDX31 antibody - N-terminal region reacts with Guinea Pig, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DDX31 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the DDX31 ddx31 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: QASSEAPPAK RRNETSFLPA KKTSVKETQR TFKGNAQKMF SPKKHSVSTS. It is sometimes possible for the material contained within the vial of "DDX31, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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