Rabbit anti-Human DDX23 Polyclonal Antibody | anti-DDX23 antibody

DDX23, ID (DDX23, Probable ATP-dependent RNA helicase DDX23, 100kD U5 snRNP-specific protein, DEAD box protein 23, PRP28 homolog, U5-100kD) (APC)

Cat. Num. AAA6291399

• Gene Names: DDX23; prp28; PRPF28; U5-100K; SNRNP100; U5-100KD
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DDX23; Polyclonal Antibody; ID (DDX23; Probable ATP-dependent RNA helicase DDX23; 100kD U5 snRNP-specific protein; DEAD box protein 23; PRP28 homolog; U5-100kD) (APC); U5-100kD; anti-DDX23 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-DDX23 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DDX23 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 255~285 amino acids from the Central region of human DDX23.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DDX23 Antibody (Center) in 293 cell line lysates (35ug/lane). DDX23 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(DDX23 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DDX23 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(DDX23 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-DDX23 antibody

DDX23 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing.

Product Categories/Family for anti-DDX23 antibody

Antibodies; DEAD Box Proteins

References

Mathew, R., et al. Nat. Struct. Mol. Biol. 15(5):435-443(2008). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 160385708
• NCBI GeneID: 9416
• UniProt Accession #: Q9BUQ8; O43188; B2R600; B4DH15
• Molecular Weight: 820
• NCBI Official Full Name: Probable ATP-dependent RNA helicase DDX23
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box polypeptide 23
• NCBI Official Symbol: DDX23
• NCBI Official Synonym Symbols: prp28; PRPF28; U5-100K; SNRNP100; U5-100KD
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX23; PRP28p homolog; DEAD box protein 23; PRP28 homolog, yeast; U5 snRNP 100 kD protein; 100 kDa U5 snRNP-specific protein
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX23
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX23
• UniProt Entry Name: DDX23_HUMAN

NCBI Description

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

DDX23: Involved in pre-mRNA splicing and its phosphorylated form (by SRPK2) is required for spliceosomal B complex formation. Belongs to the DEAD box helicase family. DDX23/PRP28 subfamily.

Protein type: Helicase; RNA-binding; RNA processing; RNA splicing; EC 3.6.4.13; Spliceosome

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: nucleoplasm; mitochondrion; snRNP U5; nucleolus; plasma membrane; nucleus

Molecular Function: protein binding; ATP-dependent helicase activity; ATP binding; ATP-dependent RNA helicase activity

Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing, via transesterification reactions; cis assembly of pre-catalytic spliceosome; RNA splicing; gene expression