Rabbit anti-Human TGM3 Polyclonal Antibody | anti-TGM3 antibody

TGM3, CT (TGM3, Protein-glutamine gamma-glutamyltransferase E, Transglutaminase E, Transglutaminase-3, Protein-glutamine gamma-glutamyltransferase E 50kD catalytic chain, Protein-glutamine gamma-glutamyltransferase E 27kD non-catalytic chain) (FITC)

Cat. Num. AAA6355297

• Gene Names: TGM3; TGE; UHS2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TGM3; Polyclonal Antibody; CT (TGM3; Protein-glutamine gamma-glutamyltransferase E; Transglutaminase E; Transglutaminase-3; Protein-glutamine gamma-glutamyltransferase E 50kD catalytic chain; Protein-glutamine gamma-glutamyltransferase E 27kD non-catalytic chain) (FITC); Protein-glutamine gamma-glutamyltransferase E 27kD non-catalytic chain; anti-TGM3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-TGM3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TGM3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 538-567 amino acids from the C-terminal region of human TGM3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TGM3 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the TGM3 antibody detected the TGM3 protein (arrow).)

Related Product Information for anti-TGM3 antibody

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene consists of two polypeptide chains activated from a single precursor protein by proteolysis. The encoded protein is involved the later stages of cell envelope formation in the epidermis and hair follicle.

Product Categories/Family for anti-TGM3 antibody

Antibodies; Enzymes;  Transferase

References

Yamane, A., et al. FEBS J. 277(17):3564-3574(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Negishi, A., et al. Cancer Sci. 100(9):1605-1611(2009). Fujimoto, A., et al. J. Hum. Genet. 54(8):461-465(2009). Uemura, N., et al. Int. J. Cancer 124(9):2106-2115(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 189458821
• NCBI GeneID: 7053
• NCBI Accession #: NP_003236.3
• NCBI GenBank Nucleotide #: NM_003245.3
• UniProt Accession #: Q08188; O95933; Q32ML9; Q32MM0; A8K5N6; B2RCR6; D3DVX1
• Molecular Weight: 76,632 Da
• NCBI Official Full Name: protein-glutamine gamma-glutamyltransferase E
• NCBI Official Synonym Full Names: transglutaminase 3
• NCBI Official Symbol: TGM3
• NCBI Official Synonym Symbols: TGE; UHS2
• NCBI Protein Information: protein-glutamine gamma-glutamyltransferase E
• UniProt Protein Name: Protein-glutamine gamma-glutamyltransferase E
• Protein Family: Protein-glutamine gamma-glutamyltransferase
• UniProt Gene Name: TGM3
• UniProt Synonym Gene Names: TG(E); TGE; TGase E; TGase-3

NCBI Description

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene consists of two polypeptide chains activated from a single precursor protein by proteolysis. The encoded protein is involved the later stages of cell envelope formation in the epidermis and hair follicle. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath.