Rabbit anti-Human THY1/Cd90 Monoclonal Antibody | anti-THY1/Cd90 antibody

Anti-THY1/Cd90 Rabbit Monoclonal Antibody

Cat. Num. AAA179742

• Gene Names: THY1; CD90; CDw90
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot
• Purity: Affinity-chromatography
• Synonyms: THY1/Cd90; Monoclonal Antibody; Anti-THY1/Cd90 Rabbit Monoclonal Antibody; Thy-1 membrane glycoprotein; CDw90; Thy-1 antigen; CD90; THY1; anti-THY1/Cd90 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Purity/Purification: Affinity-chromatography
• Form/Format: Liquid
• Sequence Length: 161

• Applicable Applications for anti-THY1/Cd90 antibody: Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; ICC/IF: 1:50-1:200
• Immunogen: A synthesized peptide derived from human THY1
• Subcellular Localization: Cell membrane; Lipid-anchor, GPI-anchor.
• Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Western blot analysis of THY1 expression in human fetal brain lysate (MBS179742).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THY1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for THY1 )

Related Product Information for anti-THY1/Cd90 antibody

May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Product Categories/Family for anti-THY1/Cd90 antibody

Immunology; Adaptive Immunity; T Cells; Neuroscience; Cell Type Marker; neuron marker; synapse marker; Stem Cells; Hematopoietic Progenitors; Lymphoid; B Lymphocytic Lineage; T Lymphocytic Lineage; Myeloid; monocytic lineage

NCBI and Uniprot Product Information

• NCBI GI #: 902967471
• NCBI GeneID: 7070
• NCBI Accession #: NP_001298089.1
• NCBI GenBank Nucleotide #: NM_001311160.1
• UniProt Accession #: P04216; Q16008; Q9NSP1
• Molecular Weight: 17935 MW
• NCBI Official Full Name: thy-1 membrane glycoprotein preproprotein
• NCBI Official Synonym Full Names: Thy-1 cell surface antigen
• NCBI Official Symbol: THY1
• NCBI Official Synonym Symbols: CD90; CDw90
• NCBI Protein Information: thy-1 membrane glycoprotein
• UniProt Protein Name: Thy-1 membrane glycoprotein
• UniProt Gene Name: THY1

NCBI Description

This gene encodes a cell surface glycoprotein and member of the immunoglobulin superfamily of proteins. The encoded protein is involved in cell adhesion and cell communication in numerous cell types, but particularly in cells of the immune and nervous systems. The encoded protein is widely used as a marker for hematopoietic stem cells. This gene may function as a tumor suppressor in nasopharyngeal carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.