Rabbit DDR1 Polyclonal Antibody | anti-DDR1 antibody

DDR1 Antibody

Cat. Num. AAA9611149

• Gene Names: DDR1; CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A
• Reactivity: Human, Mouse, Rat; Predicted: Horse, Sheep, Rabbit
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: DDR1; Polyclonal Antibody; DDR1 Antibody; CAK; CD 167; CD167; CD167 antigen-like family member A; CD167a; Cell adhesion kinase; DDR 1; DDR; DDR1_HUMAN; Discoidin domain receptor; Discoidin domain receptor tyrosine kinase 1; Discoidin receptor tyrosine kinase; Discoidin receptor tyrosine kinase isoform a; EDDR 1; EDDR1; Epithelial discoidin domain receptor 1; Epithelial discoidin domain-containing receptor 1; Epithelial specific receptor kinase; HGK2; Mammarian carcinoma kinase 10; Mammary carcinoma kinase 10; MCK-10; MCK10; NEP; Neuroepithelial tyrosine kinase; Neurotrophic tyrosine kinase receptor type 4; NTRK 4; NTRK4; OTTHUMP00000029343; OTTHUMP00000029344; OTTHUMP00000029345; OTTHUMP00000029346; OTTHUMP00000029347; OTTHUMP00000164863; OTTHUMP00000164867; OTTHUMP00000222080; Protein-tyrosine kinase 3A; Protein-tyrosine kinase RTK-6; PTK 3; PTK 3A protein tyrosine kinase 3A; PTK3; PTK3A; Receptor tyrosine kinase NEP; RTK 6; RTK6; TRK E; TRKE; Tyrosine kinase DDR; Tyrosine kinase receptor E; Tyrosine-protein kinase CAK; anti-DDR1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Horse, Sheep, Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DDR1 Antibody detects endogenous levels of DDR1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 508

• Applicable Applications for anti-DDR1 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA (Peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human DDR1.
• Tissue Specificity: Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin.
• Subcellular Location: Plasma Membrane; Plasma Membrane; Extracellular Region or Secreted; Plasma Membrane
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of DDR1 in lysates of HepG2 , using DDR1 Antibody.)

Related Product Information for anti-DDR1 antibody

Description: Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing (By similarity). Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11.

Post Translational Modifications: Autophosphorylated in response to fibrillar collagen binding. Glycosylation of Asn-211, but apparently not of Asn-260, Asn-371, or Asn-394, prevents autophosphorylation from occurring in the absence of collagen.

Subunit Structure: Homodimer. Interacts (via PPxY motif) with WWC1 (via WW domains) in a collagen-regulated manner. Forms a tripartite complex with WWC1 and PRKCZ, but predominantly in the absence of collagen. Interacts (tyrosine phosphorylated) with SHC1. Interacts with SRC. Interacts with MYH9. Interacts with CDH1. Interacts with PTPN11. Interacts with NCK2.

Similarity: The Gly/Pro-rich domains may be required for an unusual geometry of interaction with ligand or substrates. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 321400059
• NCBI GeneID: 780
• NCBI Accession #: NP_001189450.1
• NCBI GenBank Nucleotide #: NP_001189450.1
• UniProt Accession #: Q08345
• Molecular Weight: Observed: 101 kDa; Predicted: 102 kDa
• NCBI Official Full Name: epithelial discoidin domain-containing receptor 1 isoform 4
• NCBI Official Synonym Full Names: discoidin domain receptor tyrosine kinase 1
• NCBI Official Symbol: DDR1
• NCBI Official Synonym Symbols: CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A
• NCBI Protein Information: epithelial discoidin domain-containing receptor 1
• UniProt Protein Name: Epithelial discoidin domain-containing receptor 1
• Protein Family: Epithelial discoidin domain-containing receptor
• UniProt Gene Name: DDR1
• UniProt Synonym Gene Names: CAK; EDDR1; NEP; NTRK4; PTK3A; RTK6; TRKE; Epithelial discoidin domain receptor 1; MCK-10
• UniProt Entry Name: DDR1_HUMAN

NCBI Description

Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene belongs to a subfamily of tyrosine kinase receptors with homology to Dictyostelium discoideum protein discoidin I in their extracellular domain, and that are activated by various types of collagen. Expression of this protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

DDR1: Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing. Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11. Interacts (via PPxY motif) with WWC1 (via WW domains) in a collagen-regulated manner. Forms a tripartite complex with WWC1 and PRKCZ, but predominantly in the absence of collagen. Interacts (tyrosine phosphorylated) with SHC1. Interacts with SRC. Interacts with MYH9. Interacts with CDH1. Interacts with PTPN11. Interacts with NCK2. Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells. Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.10.1; Protein kinase, tyrosine (receptor); Kinase, protein; Protein kinase, TK; Membrane protein, integral; TK group; DDR family

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane; receptor complex

Molecular Function: collagen binding; protein binding; metal ion binding; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: lactation; negative regulation of cell proliferation; extracellular matrix organization and biogenesis; regulation of cell-matrix adhesion; protein amino acid autophosphorylation; smooth muscle cell migration; ear development; regulation of cell growth; cell adhesion; embryo implantation