Rabbit anti-Human CYP11B2 Polyclonal Antibody | anti-CYP11B2 antibody

CYP11B2 Antibody (Center)

Cat. Num. AAA9208915

• Gene Names: CYP11B2; CPN2; ALDOS; CYP11B; CYP11BL; CYPXIB2; P450C18; P-450C18; P450aldo
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: CYP11B2; Polyclonal Antibody; CYP11B2 Antibody (Center); Cytochrome P450 11B2; mitochondrial; Aldosterone synthase; ALDOS; Aldosterone-synthesizing enzyme; CYPXIB2; Cytochrome P-450Aldo; Cytochrome P-450C18; Steroid 18-hydroxylase; anti-CYP11B2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This CYP11B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-147 amino acids from the Central region of human CYP11B2.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.45 (varies by lot)
• Sequence Positions: 120-147
• Sequence Length: 503

• Applicable Applications for anti-CYP11B2 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:2000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-CYP11B2 Antibody (Center)at 1:2000 dilution + CEM whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 58 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(CYP11B2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYP11B2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CYP11B2 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CYP11B2 antibody

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane. The enzyme has steroid 18-hydroxylase activity to synthesize aldosterone and 18-oxocortisol as well as steroid 11 beta-hydroxylase activity. Mutations in this gene cause corticosterone methyl oxidase deficiency.

Product Categories/Family for anti-CYP11B2 antibody

Cardiovascular; Cell Biology; Metabolism; Signal Transduction

References

Wang, B., et al. Urology 76 (4), 1018 (2010) : Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) Huriletemuer, H., et al. Neurosciences (Riyadh) 15(3):184-189(2010) Cheng, X., et al. Clin. Exp. Hypertens. 32(5):301-307(2010) Nelson, D.R., et al. Pharmacogenetics 14(1):1-18(2004)

NCBI and Uniprot Product Information

• NCBI GI #: 119829183
• NCBI GeneID: 1585
• NCBI Accession #: NP_000489.3
• NCBI GenBank Nucleotide #: NM_000498.3
• UniProt Accession #: P19099; Q16726; B0ZBE4
• Molecular Weight: 57560
• NCBI Official Full Name: cytochrome P450 11B2, mitochondrial
• NCBI Official Synonym Full Names: cytochrome P450, family 11, subfamily B, polypeptide 2
• NCBI Official Symbol: CYP11B2
• NCBI Official Synonym Symbols: CPN2; ALDOS; CYP11B; CYP11BL; CYPXIB2; P450C18; P-450C18; P450aldo
• NCBI Protein Information: cytochrome P450 11B2, mitochondrial
• UniProt Protein Name: Cytochrome P450 11B2, mitochondrial
• Protein Family: Cytochrome
• UniProt Gene Name: CYP11B2
• UniProt Synonym Gene Names: ALDOS
• UniProt Entry Name: C11B2_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane. The enzyme has steroid 18-hydroxylase activity to synthesize aldosterone and 18-oxocortisol as well as steroid 11 beta-hydroxylase activity. Mutations in this gene cause corticosterone methyl oxidase deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP11B2: Preferentially catalyzes the conversion of 11- deoxycorticosterone to aldosterone via corticosterone and 18- hydroxycorticosterone. Defects in CYP11B2 are the cause of corticosterone methyloxidase type 1 deficiency (CMO-1 deficiency); also known as aldosterone deficiency due to defect in 18- hydroxylase or aldosterone deficiency I. CMO-1 deficiency is an autosomal recessive disorder of aldosterone biosynthesis. There are two biochemically different forms of selective aldosterone deficiency be termed corticosterone methyloxidase (CMO) deficiency type 1 and type 2. In CMO-1 deficiency, aldosterone is undetectable in plasma, while its immediate precursor, 18- hydroxycorticosterone, is low or normal. Defects in CYP11B2 are the cause of corticosterone methyloxidase type 2 deficiency (CMO-2 deficiency). CMO-2 is an autosomal recessive disorder of aldosterone biosynthesis. In CMO-2 deficiency, aldosterone can be low or normal, but at the expense of increased secretion of 18- hydroxycorticosterone. Consequently, patients have a greatly increased ratio of 18-hydroxycorticosterone to aldosterone and a low ratio of corticosterone to 18-hydroxycorticosterone in serum. Defects in CYP11B2 are a cause of familial hyperaldosteronism type 1 (FH1). It is a disorder characterized by hypertension, variable hyperaldosteronism, and abnormal adrenal steroid production, including 18-oxocortisol and 18-hydroxycortisol. There is significant phenotypic heterogeneity, and some individuals never develop hypertension. The molecular defect causing hyperaldosteronism familial type 1 is an anti-Lepore-type fusion of the CYP11B1 and CYP11B2 genes. The hybrid gene has the promoting part of CYP11B1, ACTH-sensitive, and the coding part of CYP11B2. Belongs to the cytochrome P450 family.

Protein type: EC 1.14.15.4; Mitochondrial; Lipid Metabolism - androgen and estrogen; Oxidoreductase; Lipid Metabolism - C21-steroid hormone; EC 1.14.15.5

Chromosomal Location of Human Ortholog: 8q21-q22

Cellular Component: mitochondrion; mitochondrial inner membrane

Molecular Function: corticosterone 18-monooxygenase activity; steroid 11-beta-monooxygenase activity; iron ion binding; heme binding

Biological Process: steroid metabolic process; xenobiotic metabolic process; mineralocorticoid biosynthetic process; C21-steroid hormone biosynthetic process; renal water homeostasis; sodium ion homeostasis; aldosterone mediated regulation of blood volume; potassium ion homeostasis; sterol metabolic process; aldosterone biosynthetic process; cellular response to hormone stimulus

Disease: Corticosterone Methyloxidase Type Ii Deficiency; Corticosterone Methyloxidase Type I Deficiency