Rabbit anti-Human DDIT4 Polyclonal Antibody | anti-DDIT4 antibody

DDIT4 Polyclonal Antibody

Cat. Num. AAA3009638

• Gene Names: DDIT4; Dig2; REDD1; REDD-1
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: DDIT4; Polyclonal Antibody; DDIT4 Polyclonal Antibody; Dig2; REDD1; REDD-1; anti-DDIT4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DDIT4 polyclonal antibody detects endogenous levels of DDIT4 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 232

• Applicable Applications for anti-DDIT4 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant protein of human DDIT4
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using DDIT4 antibody.)

Western Blot (WB)

(Western Blot analysis of extracts from normal (control) and DDIT4 knockout (KO) HeLa cells, using DDIT4 antibody.)

Related Product Information for anti-DDIT4 antibody

Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type; it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes (By similarity). Required for mTORC1-mediated defense against viral protein synthesis and virus replication (By similarity). Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death.

NCBI and Uniprot Product Information

• NCBI GI #: 9506687
• NCBI GeneID: 54541
• NCBI Accession #: NP_061931.1
• NCBI GenBank Nucleotide #: NP_061931.1
• UniProt Accession #: Q9NX09
• Molecular Weight: ~ 35kDa
• NCBI Official Full Name: DNA damage-inducible transcript 4 protein
• NCBI Official Synonym Full Names: DNA damage inducible transcript 4
• NCBI Official Symbol: DDIT4
• NCBI Official Synonym Symbols: Dig2; REDD1; REDD-1
• NCBI Protein Information: DNA damage-inducible transcript 4 protein
• UniProt Protein Name: DNA damage-inducible transcript 4 protein
• Protein Family: DNA damage-inducible transcript 4 protein
• UniProt Gene Name: DDIT4
• UniProt Synonym Gene Names: REDD1; RTP801; REDD-1
• UniProt Entry Name: DDIT4_HUMAN

Uniprot Description

DDIT4: Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Promotes neuronal cell death. Up-regulated in fibroblasts upon ionizing radiation, via a TP53-dependent pathway. Up-regulated by TP63 in primary keratinocytes, and down-regulated during keratinocyte differentiation. Up-regulated upon DNA alkylation. Up-regulated by amyloid beta-peptide and retinoic acid. Up-regulated by hypoxia, via a PI3K and HIF1A-dependent but TP53/TP63-independent mechanism. Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients. Belongs to the DDIT4 family.

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: mitochondrion; cytoplasm; intracellular; cytosol

Biological Process: neuron differentiation; cell proliferation; negative regulation of TOR signaling pathway; protein complex disassembly; nerve growth factor receptor signaling pathway; neuron migration; response to hypoxia; negative regulation of peptidyl-serine phosphorylation; brain development; negative regulation of glycolysis; defense response to virus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis