Rabbit anti-Human GLYAT Polyclonal Antibody | anti-GLYAT antibody

GLYAT, ID (GLYAT, ACGNAT, CAT, GAT, Glycine N-acyltransferase, Acyl-CoA:glycine N-acyltransferase, Aralkyl acyl-CoA N-acyltransferase, Aralkyl acyl-CoA:amino acid N-acyltransferase, Benzoyl-coenzyme A:glycine N-acyltransferase, Glycine N-benzoyltransferas

Cat. Num. AAA6302745

• Gene Names: GLYAT; CAT; GAT; ACGNAT
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GLYAT; Polyclonal Antibody; ID (GLYAT; ACGNAT; CAT; GAT; Glycine N-acyltransferase; Acyl-CoA:glycine N-acyltransferase; Aralkyl acyl-CoA N-acyltransferase; Aralkyl acyl-CoA:amino acid N-acyltransferase; Benzoyl-coenzyme A:glycine N-acyltransferase; Glycine N-benzoyltransferas; Glycine N-benzoyltransferase; HRP-1(CLP); anti-GLYAT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 296

• Applicable Applications for anti-GLYAT antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GLYAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-200 amino acids from the Central region of human GLYAT.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GLYAT Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the GLYAT antibody detected the GLYAT protein (arrow).)

Immunohistochemistry (IHC)

(GLYAT Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GLYAT Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-GLYAT antibody

The glycine-N-acyltransferase protein conjugates glycine with acyl-CoA substrates in the mitochondria. The protein is thought to be important in the detoxification of endogenous and xenobiotic acyl-CoA's. Two transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-GLYAT antibody

Antibodies; Enzymes; Acyltransferase

References

Yamamoto, A., et al. Drug Metab. Pharmacokinet. 24(1):114-117(2009). Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006). van der Westhuizen, F.H., et al. J. Biochem. Mol. Toxicol. 14(2):102-109(2000). Mawal, Y., et al. J. Pediatr. 130(6):1003-1007(1997). Merkler, D.J., et al. Arch. Biochem. Biophys. 330(2):430-434(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 111038137
• NCBI GeneID: 10249
• NCBI Accession #: NP_964011.2; NP_005829.3
• NCBI GenBank Nucleotide #: NP_964011.2
• UniProt Accession #: Q6IB77
• NCBI Official Full Name: glycine N-acyltransferase isoform a
• NCBI Official Synonym Full Names: glycine-N-acyltransferase
• NCBI Official Symbol: GLYAT
• NCBI Official Synonym Symbols: CAT; GAT; ACGNAT
• NCBI Protein Information: glycine N-acyltransferase
• UniProt Protein Name: Glycine N-acyltransferase
• Protein Family: Glycine N-acyltransferase
• UniProt Gene Name: GLYAT
• UniProt Synonym Gene Names: ACGNAT; CAT; GAT; AAc
• UniProt Entry Name: GLYAT_HUMAN

NCBI Description

The glycine-N-acyltransferase protein conjugates glycine with acyl-CoA substrates in the mitochondria. The protein is thought to be important in the detoxification of endogenous and xenobiotic acyl-CoA's. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GLYAT: Mitochondrial acyltransferase which transfers an acyl group to the N-terminus of glycine and glutamine, although much less efficiently. Can conjugate numerous substrates to form a variety of N-acylglycines, with a preference for benzoyl-CoA over phenylacetyl-CoA as acyl donors. Thereby detoxify xenobiotics, such as benzoic acid or salicylic acid, and endogenous organic acids, such as isovaleric acid. Belongs to the glycine N-acyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.1.13; Mitochondrial; EC 2.3.1.71; Transferase

Chromosomal Location of Human Ortholog: 11q12.1

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: glycine N-benzoyltransferase activity; glycine N-acyltransferase activity; transferase activity, transferring acyl groups

Biological Process: glycine metabolic process; acyl-CoA metabolic process; response to toxin; xenobiotic metabolic process; monocarboxylic acid metabolic process