Rabbit anti-Human, Mouse ATG2B Polyclonal Antibody | anti-ATG2B antibody

ATG2B, NT (ATG2B, C14orf103, Autophagy-related protein 2 homolog B) (Azide free) (HRP)

Cat. Num. AAA6276066

• Gene Names: ATG2B; C14orf103
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ATG2B; Polyclonal Antibody; NT (ATG2B; C14orf103; Autophagy-related protein 2 homolog B) (Azide free) (HRP); Autophagy-related protein 2 homolog B; anti-ATG2B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ATG2B. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ATG2B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 118-146 in the N-terminal region of human ATG2B, UniProt Accession #Q96BY7.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in CEM cell line lysates (35ug/lane) usingMBS6013465. This demonstrates that MBS6013465 detected the ATG2B protein (arrow).)

Western Blot (WB)

(Western Blot analysis in mouse Neuro-2a cell line lysates (35ug/lane) usingMBS6013465. This demonstrates that MBS6013465 detected the ATG2B protein (arrow).)

Related Product Information for anti-ATG2B antibody

The specific function of the protein remains unknown.

Product Categories/Family for anti-ATG2B antibody

Antibodies; Autophagy

References

1. Kang, M.R., et al. J. Pathol. 217(5):702-706(2009). 2. Xie, H., et al. Genomics 83(4):572-576(2004). 3. Heilig, R., et al. Nature 421(6923):601-607(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 118197272
• NCBI GeneID: 55102
• NCBI Accession #: NP_060506.5
• NCBI GenBank Nucleotide #: NM_018036.5
• UniProt Accession #: Q96BY7; Q6ZRE7; Q96DQ3; Q9NW80
• Molecular Weight: 232,763 Da
• NCBI Official Full Name: autophagy-related protein 2 homolog B
• NCBI Official Synonym Full Names: autophagy related 2B
• NCBI Official Symbol: ATG2B
• NCBI Official Synonym Symbols: C14orf103
• NCBI Protein Information: autophagy-related protein 2 homolog B; ATG2 autophagy related 2 homolog B
• UniProt Protein Name: Autophagy-related protein 2 homolog B
• Protein Family: Autophagy-related protein
• UniProt Gene Name: ATG2B
• UniProt Synonym Gene Names: C14orf103
• UniProt Entry Name: ATG2B_HUMAN

Uniprot Description

ATG2B: Required for both autophagosome formation and regulation of lipid droplet morphology and dispersion. Belongs to the ATG2 family.

Protein type: Autophagy

Chromosomal Location of Human Ortholog: 14q32.2

Cellular Component: extrinsic to membrane; pre-autophagosomal structure membrane; lipid particle

Biological Process: mitochondrion degradation; autophagic vacuole formation; cellular response to nitrogen starvation