Western Blot (WB)
(All lanes : Anti-DBF4 Antibody at 1:1000 dilutionLane 1: NIH/3T3 whole cell lysatesLane 2: HepG2 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 77 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human DBF4 Polyclonal Antibody | anti-DBF4 antibody
DBF4 Antibody
Gene Names
DBF4; ASK; CHIF; DBF4A; ZDBF1
Reactivity
Human
Applications
Western Blot, ELISA, Immunocytochemistry, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DBF4; Polyclonal Antibody; DBF4 Antibody; Protein DBF4 homolog A; Activator of S phase kinase; Chiffon homolog A; DBF4-type zinc finger-containing protein 1; ASK; DBF4A; ZDBF1; anti-DBF4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
Concentration
1mg/ml (varies by lot)
Sequence Positions
11-70
Sequence Length
234
Applicable Applications for anti-DBF4 antibody
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC)-Paraffin
Application Notes
WB~~ 1:1000
Antigen Type
synthetic peptide
Antigen Source
KLH conjugated synthetic peptide derived from human DBF4
Preparation and Storage
Store at -20 degree C.Stable for 12 months from date of receipt
Western Blot (WB)
(All lanes : Anti-DBF4 Antibody at 1:1000 dilutionLane 1: NIH/3T3 whole cell lysatesLane 2: HepG2 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 77 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-DBF4 Antibody at 1:1000 dilutionLane 1: NIH/3T3 whole cell lysatesLane 2: HepG2 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 77 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-DBF4 antibody
Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
Product Categories/Family for anti-DBF4 antibody
References
Kumagai H.,et al.Mol. Cell. Biol. 19:5083-5095(1999).
Jiang W.,et al.EMBO J. 18:5703-5713(1999).
Hollingsworth R.,et al.Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 424:157-164(2003).
Jiang W.,et al.EMBO J. 18:5703-5713(1999).
Hollingsworth R.,et al.Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 424:157-164(2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
76858
NCBI Official Full Name
protein DBF4 homolog A
NCBI Official Synonym Full Names
DBF4 zinc finger
NCBI Official Symbol
DBF4
NCBI Official Synonym Symbols
ASK; CHIF; DBF4A; ZDBF1
NCBI Protein Information
protein DBF4 homolog A
UniProt Protein Name
Protein DBF4 homolog A
Protein Family
UniProt Gene Name
DBF4
UniProt Synonym Gene Names
ASK; DBF4A; ZDBF1
UniProt Entry Name
DBF4A_HUMAN
Uniprot Description
DBF4: DBF4
Protein type: Protein kinase, regulatory subunit; Cell cycle regulation
Chromosomal Location of Human Ortholog: 7q21.3
Cellular Component: nucleoplasm
Molecular Function: nucleic acid binding; zinc ion binding; enzyme activator activity
Biological Process: positive regulation of catalytic activity; mitotic cell cycle; DNA replication; G1/S transition of mitotic cell cycle
Research Articles on DBF4
Similar Products
Product Notes
The DBF4 dbf4 (Catalog #AAA9204412) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 11-70. The DBF4 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DBF4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC)-Paraffin. WB~~ 1:1000. Researchers should empirically determine the suitability of the DBF4 dbf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DBF4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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