Rabbit anti-Human, Mouse NFAT4 Polyclonal Antibody | anti-NFAT4 antibody

NFAT4 Polyclonal Antibody

Cat. Num. AAA3009877

• Gene Names: NFATC3; NFAT4; NFATX; NF-AT4c
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: NFAT4; Polyclonal Antibody; NFAT4 Polyclonal Antibody; Nuclear factor of activated T-cells; cytoplasmic 3; NF-ATc3; NFATc3; NFATx; T-cell transcription factor NFAT4; NF-AT4; NFATC3; anti-NFAT4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NFAT4 polyclonal antibody detects endogenous levels of NFAT4 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1068

• Applicable Applications for anti-NFAT4 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant protein of human NFATC3
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using NFATC3 antibody at 1:1000 dilution.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using NFATC3 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-NFAT4 antibody

NFATc4 (nuclear factor of activated T cells, cytoplasmic, calcineurindependent 4) is a member of the nuclear factors of activated T cells DNAbinding transcription complex that influences cytokine gene expression, cardiac hypertrophy and adipocyte differentiation. This complex consists of at least two components, a cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate in the formation of this complex. NFATc4 plays a role in the inducible expression of cytokine genes in T cells, including the induction of IL-2 and IL-4. p38 MAP kinase phosphorylates multiple residues in the NFAT homology domain of NFATc4.

NCBI and Uniprot Product Information

• NCBI GI #: 4758804
• NCBI GeneID: 4775
• NCBI Accession #: NP_004546.1
• NCBI GenBank Nucleotide #: NP_004546.1
• UniProt Accession #: Q12968
• Molecular Weight: 150kDa
• NCBI Official Full Name: nuclear factor of activated T-cells, cytoplasmic 3 isoform 2
• NCBI Official Synonym Full Names: nuclear factor of activated T cells 3
• NCBI Official Symbol: NFATC3
• NCBI Official Synonym Symbols: NFAT4; NFATX; NF-AT4c
• NCBI Protein Information: nuclear factor of activated T-cells, cytoplasmic 3
• UniProt Protein Name: Nuclear factor of activated T-cells, cytoplasmic 3
• UniProt Gene Name: NFATC3
• UniProt Synonym Gene Names: NFAT4; NF-ATc3; NFATc3; NF-AT4
• UniProt Entry Name: NFAC3_HUMAN

NCBI Description

The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

NFAT4: a transcription factor that plays a role in the inducible expression of cytokine genes in T cells. Plays a role in the regulation of gene expression in T cells and immature thymocytes. Six spliced isoforms have been identified. Isoform 1 is predominantly expressed in thymus, peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 16q22.2

Cellular Component: nucleoplasm; cytoplasm; nucleolus; cytosol; nucleus

Molecular Function: protein binding; chromatin binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; patterning of blood vessels; heart development; cellular respiration; muscle cell development; innate immune response; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; inflammatory response