Rabbit Dapk3 Polyclonal Antibody | anti-Dapk3 antibody

Mouse Dapk3 Antibody (Center)

Cat. Num. AAA9204223

• Gene Names: Dapk3; dlk; ZIPK
• Reactivity: Mouse (Predicted Reactivity: Rat)
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Dapk3; Polyclonal Antibody; Mouse Dapk3 Antibody (Center); Death-associated protein kinase 3; DAP kinase 3; DAP-like kinase; Dlk; MYPT1 kinase; ZIP-kinase; Zipk; anti-Dapk3 antibody

• Host: Rabbit
• Reactivity: Mouse (Predicted Reactivity: Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Mouse Dapk3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 255-282 amino acids from the Central region of mouse Dapk3.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 255-282
• Sequence Length: 448

• Applicable Applications for anti-Dapk3 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: MOUSE
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Mouse Dapk3 Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the Dapk3 antibody detected the Dapk3 protein (arrow).)

Immunohistochemistry (IHC)

(Mouse Dapk3 Antibody (Center) (MBS9204223)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Dapk3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-Dapk3 antibody

Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection (By similarity).

Product Categories/Family for anti-Dapk3 antibody

Cancer; Cell Biology; Signal transduction

References

Chang, A.N., et al. J. Biol. Chem. 285(8):5122-5126(2010)
Ohbayashi, N., et al. Biochem. Biophys. Res. Commun. 372(4):708-712(2008)
Wooldridge, A.A., et al. J. Biol. Chem. 283(17):11850-11859(2008)
Shoval, Y., et al. PLoS Genet. 3(10):1884-1893(2007)
Sato, N., et al. Int. Immunol. 17(12):1543-1552(2005)

NCBI and Uniprot Product Information

• NCBI GI #: 299758494
• NCBI GeneID: 13144
• NCBI Accession #: NP_001177402.1; NP_031854.1
• NCBI GenBank Nucleotide #: NM_001190473.1
• UniProt Accession #: O54784
• Molecular Weight: 51422
• NCBI Official Full Name: death-associated protein kinase 3 isoform b
• NCBI Official Synonym Full Names: death-associated protein kinase 3
• NCBI Official Symbol: Dapk3
• NCBI Official Synonym Symbols: dlk; ZIPK
• NCBI Protein Information: death-associated protein kinase 3
• UniProt Protein Name: Death-associated protein kinase 3
• Protein Family: Death-associated protein kinase
• UniProt Gene Name: Dapk3
• UniProt Synonym Gene Names: Zipk; DAP kinase 3; Dlk
• UniProt Entry Name: DAPK3_MOUSE

Uniprot Description

DAPK3: Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, actin cytoskeleton reorganization, cell motility, smooth muscle contraction, and mitosis, particularly cytokinesis. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase- dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Regulates myosin phosphorylation in both smooth muscle and non- muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A, and the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Phosphorylates STAT3 and enhances its transcriptional activity. Positively regulates the canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Can disrupt the NLK-TCF7L2 complex thereby influencing the phosphorylation of TCF7L2 by NLK. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection. Monomer and homotrimer. Can also exist as homodimer or form heterodimers with ATF4. Homodimerization is required for activation segment autophosphorylation Both interactions require an intact leucine zipper domain and oligomerization is required for full enzymatic activity. Also binds to DAXX and PAWR, possibly in a ternary complex which plays a role in caspase activation. According to PubMed:17953487, does not interact with PARW. Interacts with AATF, CDC5L, UBE2D1, UBE2D2 AND UBE2D3. Interacts with AR and this interaction is enhanced by AATF. Interacts (via leucine zipper) with TCP10L (via leucine zipper). Interacts (via kinase domain) with DAPK1 (via kinase domain).Interacts with STAT3, NLK and TCF7L2. Isoform 1 and isoform 2 can interact with myosin and PPP1R12A. Isoform 2 is expressed in the bladder smooth muscle. Inhibited by pyridone 6 (K00225), a potent, ATP-competitive inhibitor. Phosphorylation at Thr-180, Thr-225 and Thr-265 is essential for activity. Oligomerization is required for full enzymatic activity. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Tumor suppressor; CAMK group; DAPK family

Cellular Component: PML body; cytoskeleton; cytoplasm; chromosome; nucleus; chromosome, pericentric region; lipid raft

Molecular Function: identical protein binding; protein homodimerization activity; Rho GTPase binding; calmodulin-dependent protein kinase activity; nucleotide binding; protein kinase activity; transferase activity; protein serine/threonine kinase activity; leucine zipper domain binding; protein binding; transferase activity, transferring phosphorus-containing groups; kinase activity; ATP binding

Biological Process: transcription, DNA-dependent; apoptosis; positive regulation of apoptosis; protein amino acid autophosphorylation; regulation of myosin II filament assembly or disassembly; regulation of mitotic cell cycle; chromatin modification; protein amino acid phosphorylation; regulation of apoptosis; regulation of cell shape; regulation of transcription, DNA-dependent; negative regulation of translation; regulation of focal adhesion formation; phosphorylation; positive regulation of cell migration