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DAP antibody pair

DAP Antibody Pair

Reactivity
Human, Hamster, Pig, Dog, Rabbit
Applications
ELISA
Synonyms
DAP; Polyclonal Antibody Pair; DAP Antibody Pair; Death-associated protein 1; DAP-1; DAP antibody pair
Ordering
For Research Use Only!
Host
Capture: Rabbit
Detection: Rabbit
Reactivity
Human, Hamster, Pig, Dog, Rabbit
Clonality
Polyclonal
Isotype
Capture: IgG
Detection: IgG
Form/Format
Liquid
Concentration
The concentrations and volumes of the antibodies may vary and be lot specific. Details will be on the vial labels. (varies by lot)
Sequence Length
102
Applicable Applications for DAP antibody pair
Sandwich ELISA (EIA)
Application Notes
We recommend using the capture antibody at a concentration of 0.8ug/ml and the detection antibody at a concentration of 0.4ug/ml.
Immunogen Species
Human
Conjugation
Capture: Non-conjugated; Detection: Biotin conjugated
Storage Buffer
Capture: 50% Glycerol, 0.01M PBS, PH 7.4
Detection: 50% Glycerol, 0.01M PBS, PH 7.4
Other Note
Immunogen information is company proprietary information. Antibodies are sold as a pair and not available separately.
Preparation and Storage
Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze/thaw cycles. When stored properly, products should be stable for at least 5 months.

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
Molecular Weight
11,165 Da
NCBI Official Full Name
DAP, partial
UniProt Protein Name
Death-associated protein 1
Protein Family
UniProt Gene Name
DAP
UniProt Synonym Gene Names
DAP1; DAP-1
UniProt Entry Name
DAP1_HUMAN

Uniprot Description

DAP: a 15 kDa, proline rich protein that possesses one SH3 binding motif. Negatively regulates autophagy and is involved in mediating interferon-gamma-induced cell death. Localizes in the cytoplasm, but the detailed mechanism of its proapoptotic function is unclear.

Protein type: Autophagy

Chromosomal Location of Human Ortholog: 5p15.2

Biological Process: apoptosis; autophagy; caspase activation; cellular response to amino acid starvation; inhibition of NF-kappaB transcription factor; negative regulation of autophagy; negative regulation of transcription, DNA-dependent

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Product Notes

The DAP dap (Catalog #AAA7041735) is an Antibody Pair produced from Capture: Rabbit Detection: Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DAP Antibody Pair reacts with Human, Hamster, Pig, Dog, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's DAP can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (EIA). We recommend using the capture antibody at a concentration of 0.8ug/ml and the detection antibody at a concentration of 0.4ug/ml. Researchers should empirically determine the suitability of the DAP dap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DAP, Polyclonal Antibody Pair" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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