Goat anti-Human DAGLA Polyclonal Antibody | anti-Dagla antibody

DAGLA, CT (Sn1-specific Diacylglycerol Lipase alpha, DGL-alpha, Neural Stem Cell-derived Dendrite Regulator, C11orf11, KIAA0659, NSDDR)

Cat. Num. AAA6006466

• Gene Names: Dagla; Nsddr
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: DAGLA; Polyclonal Antibody; CT (Sn1-specific Diacylglycerol Lipase alpha; DGL-alpha; Neural Stem Cell-derived Dendrite Regulator; C11orf11; KIAA0659; NSDDR); Anti -DAGLA; anti-Dagla antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human DAGLA. Species sequence homology: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

• Applicable Applications for anti-Dagla antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, ELISA and Immunohistochemistry.; Dilution: ELISA: 1:128,000, 0.3-1ug/ml; Western Blot: 0.3-1ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to C-PAKQDELVISAR from human DAGLA at C-terminal.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Breast (formalin-fixed, paraffin-embedded) stained with DAGLA antibody at 5ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Immunohistochemistry (IHC)

(Human Pancreas (formalin-fixed, paraffin-embedded) stained with DAGLA antibody at 5ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Immunohistochemistry (IHC)

(Human Spleen (formalin-fixed, paraffin-embedded) stained with DAGLA antibody at 5ug/ml followed by biotinylated anti-goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Testing Data

(Antibody (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)

Related Product Information for anti-Dagla antibody

Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses.

Product Categories/Family for anti-Dagla antibody

Antibodies; Abs to Enzymes; Lipase

NCBI and Uniprot Product Information

• NCBI GI #: 133777779
• NCBI GeneID: 269060
• UniProt Accession #: Q6WQJ1; Q6ZQ76; A8WFL4
• Molecular Weight: 115,375 Da
• NCBI Official Full Name: Dagla protein
• NCBI Official Synonym Full Names: diacylglycerol lipase, alpha
• NCBI Official Symbol: Dagla
• NCBI Official Synonym Symbols: Nsddr
• NCBI Protein Information: sn1-specific diacylglycerol lipase alpha; DGL-alpha; neural stem cell-derived dendrite regulator
• UniProt Protein Name: Sn1-specific diacylglycerol lipase alpha
• Protein Family: Sn1-specific diacylglycerol lipase
• UniProt Gene Name: Dagla
• UniProt Synonym Gene Names: Kiaa0659; Nsddr; DGL-alpha
• UniProt Entry Name: DGLA_MOUSE

Uniprot Description

NSDDR: Catalyzes the hydrolysis of diacylglycerol (DAG) to 2- arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses. Defects in DAGLA may be a cause of spinocerebellar ataxia type 20 (SCA20). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA20 is an autosomal dominant, adult- onset form characterized by dysarthria due to spasmodic dysphonia followed by slowly progressive ataxia. A copy number variation consisting of a 260-kb duplication at chromosome 11q12.2-12.3 is responsible for SCA20. The critical gene within the duplicated segment may be DAGLA. Belongs to the AB hydrolase superfamily. Lipase family.

Protein type: Membrane protein, multi-pass; Hydrolase; Membrane protein, integral; EC 3.1.1.-

Cellular Component: membrane; integral to membrane; plasma membrane

Molecular Function: retinyl-palmitate esterase activity; sulfolactone hydrolase activity; 3,4-dihydrocoumarin hydrolase activity; hydrolase activity; metal ion binding; lipase activity; butyrolactone hydrolase activity; all-trans-retinyl-palmitate hydrolase activity; phospholipase activity; 1-oxa-2-oxocycloheptane lactonase activity; prenylcysteine methylesterase activity

Biological Process: neurogenesis; neuroblast proliferation; diacylglycerol catabolic process; arachidonic acid metabolic process; lipid metabolic process; lipid catabolic process; neurotransmitter biosynthetic process