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Western Blot (WB) (Western blot analysis of DAGLB Antibody (Center) in MCF-7 cell line lysates (35ug/lane). DAGLB (arrow) was detected using the purified Pab;)

Rabbit anti-Human, mouse DAGLB Polyclonal Antibody | anti-DAGLB antibody

DAGLB Antibody (Center)

Gene Names
DAGLB; KCCR13L; DAGLBETA
Reactivity
Human, mouse
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DAGLB; Polyclonal Antibody; DAGLB Antibody (Center); Sn1-specific diacylglycerol lipase beta; DGL-beta; 311-; KCCR13L; anti-DAGLB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DAGLB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 491-518 amino acids from the Central region of human DAGLB.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
491-518
Sequence Length
543
Applicable Applications for anti-DAGLB antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of DAGLB Antibody (Center) in MCF-7 cell line lysates (35ug/lane). DAGLB (arrow) was detected using the purified Pab;)

Western Blot (WB) (Western blot analysis of DAGLB Antibody (Center) in MCF-7 cell line lysates (35ug/lane). DAGLB (arrow) was detected using the purified Pab;)

Western Blot (WB)

(Western blot analysis of DAGLB Antibody (Center) in mouse cerebellum tissue lysates (35ug/lane). DAGLB (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of DAGLB Antibody (Center) in mouse cerebellum tissue lysates (35ug/lane). DAGLB (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(DAGLB Antibody (Center) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (DAGLB Antibody (Center) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-DAGLB antibody
Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses.
Product Categories/Family for anti-DAGLB antibody
References
Ma,J. Atherosclerosis 191 (1), 63-72 (2007)
Bisogno,T. J. Cell Biol. 163 (3), 463-468 (2003)
Lanfranchi,G. Genome Res. 6 (1), 35-42 (1996)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
73732
NCBI Official Full Name
sn1-specific diacylglycerol lipase beta isoform 2
NCBI Official Synonym Full Names
diacylglycerol lipase, beta
NCBI Official Symbol
DAGLB
NCBI Official Synonym Symbols
KCCR13L; DAGLBETA
NCBI Protein Information
sn1-specific diacylglycerol lipase beta
UniProt Protein Name
Sn1-specific diacylglycerol lipase beta
UniProt Gene Name
DAGLB
UniProt Synonym Gene Names
DGL-beta
UniProt Entry Name
DGLB_HUMAN

Uniprot Description

DAGLBETA: Catalyzes the hydrolysis of diacylglycerol (DAG) to 2- arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses. Belongs to the AB hydrolase superfamily. Lipase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; EC 3.1.1.-; Lipase

Chromosomal Location of Human Ortholog: 7p22.1

Cellular Component: lysosomal membrane; integral to membrane; plasma membrane

Molecular Function: metal ion binding; lipase activity

Biological Process: platelet activation; neuroblast proliferation; arachidonic acid metabolic process; blood coagulation; lipid catabolic process; neurotransmitter biosynthetic process

Research Articles on DAGLB

Similar Products

Product Notes

The DAGLB daglb (Catalog #AAA9213678) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 491-518. The DAGLB Antibody (Center) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DAGLB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the DAGLB daglb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DAGLB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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