Rabbit anti-Human, Mouse DACT1 Polyclonal Antibody | anti-DACT1 antibody

DACT1, NT (DACT1, DPR1, HNG3, Dapper homolog 1, Hepatocellular carcinoma novel gene 3 protein) (Azide free) (HRP)

Cat. Num. AAA6290709

• Gene Names: DACT1; DPR1; FRODO; HDPR1; DAPPER; THYEX3; DAPPER1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DACT1; Polyclonal Antibody; NT (DACT1; DPR1; HNG3; Dapper homolog 1; Hepatocellular carcinoma novel gene 3 protein) (Azide free) (HRP); Hepatocellular carcinoma novel gene 3 protein; anti-DACT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-DACT1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DACT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-49 amino acids from the N-terminal region of human DACT1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DACT1 Antibody (N-term) western blot analysis in mouse testis tissue lysates (15ug/lane).This demonstrates the DACT1 antibody detected DACT1 protein (arrow).)

Immunohistochemistry (IHC)

(DACT1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DACT1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(DACT1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-DACT1 antibody

Antibodies; Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 24158478
• NCBI GeneID: 51339
• NCBI Accession #: NP_057735.2
• NCBI GenBank Nucleotide #: NM_016651.5
• UniProt Accession #: Q9NYF0; Q86TY0; A8MYJ2
• Molecular Weight: 86,212 Da
• NCBI Official Full Name: dapper homolog 1 isoform 1
• NCBI Official Synonym Full Names: dishevelled-binding antagonist of beta-catenin 1
• NCBI Official Symbol: DACT1
• NCBI Official Synonym Symbols: DPR1; FRODO; HDPR1; DAPPER; THYEX3; DAPPER1
• NCBI Protein Information: dapper homolog 1; dapper antagonist of catenin 1; heptacellular carcinoma novel gene 3; dapper, antagonist of beta-catenin, homolog 1; hepatocellular carcinoma novel gene 3 protein
• UniProt Protein Name: Dapper homolog 1
• Protein Family: Dapper
• UniProt Gene Name: DACT1
• UniProt Synonym Gene Names: DPR1; HNG3; hDPR1
• UniProt Entry Name: DACT1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the dapper family, characterized by the presence of PDZ-binding motif at the C-terminus. It interacts with, and positively regulates dishevelled-mediated signaling pathways during development. Depletion of this mRNA from xenopus embryos resulted in loss of notochord and head structures, and mice lacking this gene died shortly after birth from severe posterior malformations. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

DACT1: Positively regulates DVL2-mediated signaling pathways during development. Binds to DVL2 and impedes the degradation of CTNNB1/beta-catenin, thereby enhancing the transcriptional activation of target genes of the Wnt signaling pathway. Interacts with DVL1 and DVL2. Belongs to the dapper family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 14q23.1

Cellular Component: nucleoplasm; cytoplasm; synapse; cell junction; beta-catenin destruction complex; cytosol

Molecular Function: protein binding; protein kinase C binding; protein kinase A binding; beta-catenin binding

Biological Process: negative regulation of JNK cascade; negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; dendrite morphogenesis; positive regulation of protein catabolic process; synapse organization and biogenesis; positive regulation of fat cell differentiation; regulation of protein stability; gastrulation with mouth forming second; negative regulation of transcription from RNA polymerase II promoter; positive regulation of Wnt receptor signaling pathway; embryonic hindgut morphogenesis