Western Blot (WB)
(DACT1 Antibody (N-term) western blot analysis in mouse testis tissue lysates (15ug/lane).This demonstrates the DACT1 antibody detected DACT1 protein (arrow).)
Rabbit anti-Human, Mouse DACT1 Polyclonal Antibody | anti-DACT1 antibody
DACT1, NT (DACT1, DPR1, HNG3, Dapper homolog 1, Hepatocellular carcinoma novel gene 3 protein) (APC)
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Western Blot (WB)
(DACT1 Antibody (N-term) western blot analysis in mouse testis tissue lysates (15ug/lane).This demonstrates the DACT1 antibody detected DACT1 protein (arrow).)
Western Blot (WB)
(DACT1 Antibody (N-term) western blot analysis in mouse testis tissue lysates (15ug/lane).This demonstrates the DACT1 antibody detected DACT1 protein (arrow).)
Immunohistochemistry (IHC)
(DACT1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DACT1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(DACT1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DACT1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(DACT1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(DACT1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the dapper family, characterized by the presence of PDZ-binding motif at the C-terminus. It interacts with, and positively regulates dishevelled-mediated signaling pathways during development. Depletion of this mRNA from xenopus embryos resulted in loss of notochord and head structures, and mice lacking this gene died shortly after birth from severe posterior malformations. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
Uniprot Description
DACT1: Positively regulates DVL2-mediated signaling pathways during development. Binds to DVL2 and impedes the degradation of CTNNB1/beta-catenin, thereby enhancing the transcriptional activation of target genes of the Wnt signaling pathway. Interacts with DVL1 and DVL2. Belongs to the dapper family.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 14q23.1
Cellular Component: nucleoplasm; cytoplasm; synapse; cell junction; beta-catenin destruction complex; cytosol
Molecular Function: protein binding; protein kinase C binding; protein kinase A binding; beta-catenin binding
Biological Process: negative regulation of JNK cascade; negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; dendrite morphogenesis; positive regulation of protein catabolic process; synapse organization and biogenesis; positive regulation of fat cell differentiation; regulation of protein stability; gastrulation with mouth forming second; negative regulation of transcription from RNA polymerase II promoter; positive regulation of Wnt receptor signaling pathway; embryonic hindgut morphogenesis
Research Articles on DACT1
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Product Notes
The DACT1 dact1 (Catalog #AAA6290706) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DACT1, NT (DACT1, DPR1, HNG3, Dapper homolog 1, Hepatocellular carcinoma novel gene 3 protein) (APC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DACT1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DACT1 dact1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DACT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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