Rabbit anti-Human, Mouse DACT1 Polyclonal Antibody | anti-DACT1 antibody

DACT1 Antibody

Cat. Num. AAA9629872

• Gene Names: DACT1; DPR1; FRODO; HDPR1; DAPPER; THYEX3; DAPPER1
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: DACT1; Polyclonal Antibody; DACT1 Antibody; Dact; dact1; DACT1_HUMAN; Dapper antagonist of beta catenin homolog 1; Dapper antagonist of catenin 1; Dapper; Dapper homolog 1; DAPPER1; Dpr; Dpr1; Frd; Frodo; hDPR1; Hepatocellular carcinoma novel gene 3 protein; Heptacellular carcinoma novel gene 3; HNG3; MTNG3; Thyex3; anti-DACT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: DACT1 Antibody detects endogenous levels of DACT1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-DACT1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human DACT1.
• Conjugation: Unconjugated
• Similarity: The C-terminal PDZ-binding motif mediates interaction with the PDZ domains of DSH (Dishevelled) family proteins. Belongs to the dapper family.
• Subunit Structure: Can form homodimers and heterodimers with DACT2 or DACT3. Interacts with CSNK1D, PKA catalytic subunit, PKC-type kinase, CSNK2A1, CSNK2B, DVL1, DVL3, VANGL1, VANGL2, CTNND1 and HDAC1 (By similarity). Interacts with DVL2. Interacts with YWHAB; the interaction is enhanced by PKA phosphorylating DACT1 at Ser-237 and Ser-827. Interacts with CTNNB1 and HDAC1. Interacts with GSK3B; the interaction is indicative for an association of DACT1 with the beta-catenin destruction complex. Interacts with GSK3A.
• Function: Involved in regulation of intracellular signaling pathways during development. Specifically thought to play a role in canonical and/or non-canonical Wnt signaling pathways through interaction with DSH (Dishevelled) family proteins. The activation/inhibition of Wnt signaling may depend on the phosphorylation status. Proposed to regulate the degradation of CTNNB1/beta-catenin, thereby modulating the transcriptional activation of target genes of the Wnt signaling pathway. Its function in stabilizing CTNNB1 may involve inhibition of GSK3B activity. Promotes the membrane localization of CTNNB1. The cytoplasmic form can induce DVL2 degradation via a lysosome-dependent mechanism; the function is inhibited by PKA-induced binding to 14-3-3 proteins, such as YWHAB. Seems to be involved in morphogenesis at the primitive streak by regulating VANGL2 and DVL2; the function seems to be independent of canonical Wnt signaling and rather involves the non-canonical Wnt/planar cell polarity (PCP) pathway (By similarity). The nuclear form may prevent the formation of LEF1:CTNNB1 complex and recruit HDAC1 to LEF1 at target gene promoters to repress transcription thus antagonizing Wnt signaling. May be involved in positive regulation of fat cell differentiation. During neuronal differentiation may be involved in excitatory synapse organization, and dendrite formation and establishment of spines.
• Note: huttles between the nucleus and the cytoplasm. Seems to be nuclear in the absence of Wnt signaling and to translocate to the cytoplasm in its presence.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-DACT1 antibody

Cytoplasm; Nucleus; Cell junction; Synapse; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 24158478
• NCBI GeneID: 51339
• NCBI Accession #: NP_057735.2
• NCBI GenBank Nucleotide #: NM_016651.5
• UniProt Accession #: Q9NYF0; Q86TY0; A8MYJ2
• Molecular Weight: 86,212 Da
• NCBI Official Full Name: dapper homolog 1 isoform 1
• NCBI Official Synonym Full Names: dishevelled-binding antagonist of beta-catenin 1
• NCBI Official Symbol: DACT1
• NCBI Official Synonym Symbols: DPR1; FRODO; HDPR1; DAPPER; THYEX3; DAPPER1
• NCBI Protein Information: dapper homolog 1; dapper antagonist of catenin 1; heptacellular carcinoma novel gene 3; dapper, antagonist of beta-catenin, homolog 1; hepatocellular carcinoma novel gene 3 protein
• UniProt Protein Name: Dapper homolog 1
• Protein Family: Dapper
• UniProt Gene Name: DACT1
• UniProt Synonym Gene Names: DPR1; HNG3; hDPR1
• UniProt Entry Name: DACT1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the dapper family, characterized by the presence of PDZ-binding motif at the C-terminus. It interacts with, and positively regulates dishevelled-mediated signaling pathways during development. Depletion of this mRNA from xenopus embryos resulted in loss of notochord and head structures, and mice lacking this gene died shortly after birth from severe posterior malformations. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

DACT1: Positively regulates DVL2-mediated signaling pathways during development. Binds to DVL2 and impedes the degradation of CTNNB1/beta-catenin, thereby enhancing the transcriptional activation of target genes of the Wnt signaling pathway. Interacts with DVL1 and DVL2. Belongs to the dapper family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 14q23.1

Cellular Component: nucleoplasm; cytoplasm; synapse; cell junction; beta-catenin destruction complex; cytosol

Molecular Function: protein binding; protein kinase C binding; protein kinase A binding; beta-catenin binding

Biological Process: negative regulation of JNK cascade; negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; dendrite morphogenesis; positive regulation of protein catabolic process; synapse organization and biogenesis; positive regulation of fat cell differentiation; regulation of protein stability; gastrulation with mouth forming second; negative regulation of transcription from RNA polymerase II promoter; positive regulation of Wnt receptor signaling pathway; embryonic hindgut morphogenesis