Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed A431, using Cytokeratin 2 antibody at 1:200 dilution.)
Rabbit Cytokeratin 2 Polyclonal Antibody | anti-KRT76 antibody
Cytokeratin 2 antibody
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Cytokeratin 2 antibody was purified by antigen-affinity chromatography
Synonyms
Cytokeratin 2; Polyclonal Antibody; Cytokeratin 2 antibody; Polyclonal Cytokeratin 2; Anti-Cytokeratin 2; KRT2E; KRT2A; KRTE; epithelial keratin-2e; epidermal ichthyosis bullosa of Siemens; MGC116968; MGC116967; Cytokeratin -2; cytokeratin-2e; KRT2; CK-2e; K2e; keratin-2 epidermis; anti-KRT76 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Cytokeratin 2 antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.67 mg/ml (varies by lot)
Sequence Length
638
Applicable Applications for anti-KRT76 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF:1:100-1:200
ICC:1:100-1:200
IHC-P: 1:100-1:500
WB: 1:500-1:3000
ICC:1:100-1:200
IHC-P: 1:100-1:500
WB: 1:500-1:3000
Biological Significance
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
Cross-Reactivity
Human
Immunogen
Cytokeratin 2 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 143 and 529 of Cytokeratin 2
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed A431, using Cytokeratin 2 antibody at 1:200 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed A431, using Cytokeratin 2 antibody at 1:200 dilution.)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: A431) using a 10 % SDS PAGE gel and Cytokeratin 2 antibody)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: A431) using a 10 % SDS PAGE gel and Cytokeratin 2 antibody)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded H661 xenograft using Cytokeratin 2 antibody at a dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded H661 xenograft using Cytokeratin 2 antibody at a dilution of 1:100)
Related Product Information for anti-KRT76 antibody
Affinity purified Rabbit polyclonal Cytokeratin 2 antibody
Product Categories/Family for anti-KRT76 antibody
NCBI and Uniprot Product Information
Uniprot Description
K76: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 12q13.13
Cellular Component: keratin filament; intermediate filament; nucleus
Molecular Function: structural molecule activity
Biological Process: cytoskeleton organization and biogenesis
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Product Notes
The Cytokeratin 2 krt76 (Catalog #AAA835666) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cytokeratin 2 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF:1:100-1:200 ICC:1:100-1:200 IHC-P: 1:100-1:500 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the Cytokeratin 2 krt76 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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