Immunohistochemistry (IHC)
(Human Breast (formalin-fixed, paraffin-embedded) stained with Keratin antibody at 10ug/ml followed by biotinylated mouse IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)
Mouse anti-Human Cytokeratin, Pan Monoclonal Antibody | anti-KRT76 antibody
Cytokeratin, Pan
Gene Names
KRT76; KRT2B; KRT2P; HUMCYT2A
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
Cytokeratin; Pan; Monoclonal Antibody; Anti -Cytokeratin; anti-KRT76 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
C-11
Specificity
Recognizes human Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18.. Species sequence homology: Mammalian
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 15mM sodium azide, pH7.4.
Applicable Applications for anti-KRT76 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Immunogen
Keratin-enriched preparation from human epidermoid carcinoma cell line A431.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(Human Breast (formalin-fixed, paraffin-embedded) stained with Keratin antibody at 10ug/ml followed by biotinylated mouse IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)
Immunohistochemistry (IHC)
(Human Breast (formalin-fixed, paraffin-embedded) stained with Keratin antibody at 10ug/ml followed by biotinylated mouse IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)
Related Product Information for anti-KRT76 antibody
Cytokeratin filaments consist of a complex family of ~20 different cytokeratin polypeptides with relative molecular weights of 40kD to 68kD. These intracellular intermediate filaments of 10nm in diameter provide structural integrity to epithelial cells. All the filament subunits share a general common fibrillar organization, consisting of a central alpha-helical rod that is flanked by N- and C-terminal domains at each end. Generally, low molecular weight keratins are expressed in the early stages of cell development, whereas high molecular weight keratins are expressed in maturing cells.
Product Categories/Family for anti-KRT76 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
65,841 Da
NCBI Official Full Name
cytokeratin 2
NCBI Official Synonym Full Names
keratin 76
NCBI Official Symbol
KRT76
NCBI Official Synonym Symbols
KRT2B; KRT2P; HUMCYT2A
NCBI Protein Information
keratin, type II cytoskeletal 2 oral; K2P; K76; CK-2P; keratin 2p; keratin-76; cytokeratin 2; cytokeratin-2P; type-II keratin Kb9
UniProt Protein Name
Keratin, type II cytoskeletal 2 oral
Protein Family
UniProt Gene Name
KRT76
UniProt Synonym Gene Names
KRT2B; KRT2P; CK-2P; K2P; K76
UniProt Entry Name
K22O_HUMAN
NCBI Description
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. The type II keratins are clustered in a region of chromosome 12q13. [provided by RefSeq, Jun 2009]
Uniprot Description
K76: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 12q13.13
Cellular Component: keratin filament; intermediate filament; nucleus
Molecular Function: structural molecule activity
Biological Process: cytoskeleton organization and biogenesis
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Product Notes
The KRT76 krt76 (Catalog #AAA6005242) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Cytokeratin, Pan reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin, Pan can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml. Researchers should empirically determine the suitability of the KRT76 krt76 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin, Pan, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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