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Western Blot (WB) (Western blot analysis of Cytochrome P450 4F12 expression in NCI-H460 cell line lysates, The lane on the left is treated with the antigen-specific peptide.)

Rabbit anti-Human, Mouse Cytochrome P450 4F12 Polyclonal Antibody | anti-CYP4F12 antibody

Cytochrome P450 4F12 Antibody

Gene Names
CYP4F12; CYPIVF12; F22329_1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Cytochrome P450 4F12; Polyclonal Antibody; Cytochrome P450 4F12 Antibody; CP4FC_HUMAN; CYP4F12; CYPIVF12; Cytochrome P450; family 4; subfamily F; polypeptide 12; anti-CYP4F12 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Cytochrome P450 4F12 antibody detects endogenous levels of total Cytochrome P450 4F12
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
524
Applicable Applications for anti-CYP4F12 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
Immunogen
A synthesized peptide
Subcellular Location
Endoplasmic Reticulum Membrane. Microsome Membrane.
Tissue Specificity
Expressed in small intestine, liver, colon and heart.
Conjugation
Unconjugated

Western Blot (WB)

(Western blot analysis of Cytochrome P450 4F12 expression in NCI-H460 cell line lysates, The lane on the left is treated with the antigen-specific peptide.)

Western Blot (WB) (Western blot analysis of Cytochrome P450 4F12 expression in NCI-H460 cell line lysates, The lane on the left is treated with the antigen-specific peptide.)

Immunohistochemistry (IHC)

(MBS9604128 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Immunohistochemistry (IHC) (MBS9604128 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-CYP4F12 antibody
Description: Catalyzes leukotriene B4 omega-hydroxylation and arachidonic acid omega-hydroxylation but with an activity much lower than that of CYP4F2. Catalyzes the hydroxylation of the antihistamine ebastine.
Similarity: Belongs to the cytochrome P450 family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 60 kDa
Predicted: 61 kDa
NCBI Official Full Name
cytochrome P450 4F12
NCBI Official Synonym Full Names
cytochrome P450 family 4 subfamily F member 12
NCBI Official Symbol
CYP4F12
NCBI Official Synonym Symbols
CYPIVF12; F22329_1
NCBI Protein Information
cytochrome P450 4F12
UniProt Protein Name
Cytochrome P450 4F12
Protein Family
UniProt Gene Name
CYP4F12

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein likely localizes to the endoplasmic reticulum. When expressed in yeast the enzyme is capable of oxdizing arachidonic acid. It can also catalyze the epoxidation of 22:6n-3 and 22:5n-3 polyunsaturated long-chain fatty acids. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Uniprot Description

Catalyzes leukotriene B4 omega-hydroxylation and arachidonic acid omega-hydroxylation but with an activity much lower than that of CYP4F2. Catalyzes the hydroxylation of the antihistamine ebastine.

Research Articles on CYP4F12

Similar Products

Product Notes

The CYP4F12 cyp4f12 (Catalog #AAA9604128) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cytochrome P450 4F12 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome P450 4F12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the CYP4F12 cyp4f12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome P450 4F12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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