Human Semaphorin 4D (SEMA4D) ELISA Kit | SEMA4D elisa kit

Human Semaphorin 4D (SEMA4D) ELISA Kit

Cat. Num. AAA2023012

• Gene Names: SEMA4D; CD100; SEMAJ; coll-4; C9orf164; M-sema-G
• Reactivity: Human
• Synonyms: Semaphorin 4D (SEMA4D); Human Semaphorin 4D (SEMA4D) ELISA Kit; CD100; A8; BB18; GR3; M-Sema G; M-Sema-G; SEMAJ; Coll-4; Sema Domain; Immunoglobulin Domain(Ig); Transmembrane Domain(TM)and Short Cytoplasmic Domain 4D; SEMA4D elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SEMA4D. No significant cross-reactivity or interference between SEMA4D and analogues was observed.
• Sequence Length: 862

• Samples: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.2-2,000pg/mL
• Sensitivity: < 12.1pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SEMA4D were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SEMA4D were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SEMA4D elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SEMA4D in human serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to SEMA4D. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to SEMA4D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SEMA4D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SEMA4D in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SEMA4D elisa kit

CD & Adhesion molecule; Tumor immunity; Infection immunity; Immune molecule

NCBI and Uniprot Product Information

• NCBI GI #: 214010218
• NCBI GeneID: 10507
• NCBI Accession #: NP_006369.3
• NCBI GenBank Nucleotide #: NM_006378.3
• Molecular Weight: 82,164 Da
• NCBI Official Full Name: semaphorin-4D isoform 1
• NCBI Official Synonym Full Names: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
• NCBI Official Symbol: SEMA4D
• NCBI Official Synonym Symbols: CD100; SEMAJ; coll-4; C9orf164; M-sema-G
• NCBI Protein Information: semaphorin-4D
• UniProt Protein Name: Semaphorin-4D
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA4D
• UniProt Synonym Gene Names: C9orf164; CD100; SEMAJ
• UniProt Entry Name: SEM4D_HUMAN

Uniprot Description

SEMA4D: a single-pass type I membrane protein that my play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q22.2

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; receptor activity; semaphorin receptor binding; receptor binding

Biological Process: negative regulation of peptidyl-tyrosine phosphorylation; axon guidance; regulation of dendrite morphogenesis; negative regulation of cell adhesion; negative regulation of transcription from RNA polymerase II promoter; positive regulation of phosphoinositide 3-kinase cascade; regulation of cell shape; positive regulation of peptidyl-tyrosine phosphorylation; immune response; negative regulation of osteoblast differentiation; positive regulation of protein amino acid phosphorylation; cell adhesion; positive regulation of collateral sprouting; negative regulation of apoptosis; regulation of cell projection organization and biogenesis; positive regulation of cell migration