Western Blot (WB)
(Anti-CYP2E1 antibody, MBS176396, Western blottingLane 1: Rat Liver Tissue LysateLane 2: Mouse Liver Tissue Lysate )
Rabbit anti-Mouse, Rat Cytochrome P450 2E1 Polyclonal Antibody | anti-CYP2E1 antibody
Anti-Cytochrome P450 2E1 antibody
Western Blot (WB)
(Anti-CYP2E1 antibody, MBS176396, Western blottingLane 1: Rat Liver Tissue LysateLane 2: Mouse Liver Tissue Lysate )
Western Blot (WB)
(Anti-CYP2E1 antibody, MBS176396, Western blottingLane 1: Rat Liver Tissue LysateLane 2: Mouse Liver Tissue Lysate )
Immunohistochemistry (IHC)
(Anti-CYP2E1 antibody, MBS176396, IHC(P)IHC(P): Rat Liver Tissue )
Immunohistochemistry (IHC)
(Anti-CYP2E1 antibody, MBS176396, IHC(P)IHC(P): Rat Liver Tissue )
Background: CYP2E1, also known as P450IIE1, is a member of the P450IIE subfamily which is ethanol-inducible. It has at least 1 gene which is mapped to 10q24.3-qter, and a second is likely in rat and in man. Both the rat and human proteins encoded by this gene contain 493 amino acids and calculated molecular masses of 56,634 and 56,916 daltons, respectively. In addition, genetic polymorphisms in the 5-prime flanking region of the human P450IIE1 gene affected its binding of transacting factor and changed its transcriptional regulation, which may lead to interindividual differences of microsomal drug oxidation activity. P450IIE1 is an important enzyme for the catalysis of the conversion of ethanol to acetaldehyde and to acetate in humans,and it is also involved in the metabolism of nitrosamines. Due to the possible correlation of P450IIE1 genes with malignancy, clinical studies of RFLP patterns of these genes in cancer may be useful.
NCBI and Uniprot Product Information
Uniprot Description
CYP2E1: Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. Belongs to the cytochrome P450 family.
Protein type: Oxidoreductase; Lipid Metabolism - arachidonic acid; Lipid Metabolism - linoleic acid; Endoplasmic reticulum; EC 1.14.13.n7; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Mitochondrial; Xenobiotic Metabolism - metabolism by cytochrome P450
Cellular Component: Golgi membrane; intrinsic to endoplasmic reticulum membrane; intracellular membrane-bound organelle; membrane; mitochondrion; endoplasmic reticulum
Molecular Function: 3-(3-hydroxy-phenyl)propionate hydroxylase activity; toluene-sulfonate methyl-monooxygenase activity; xylene monooxygenase activity; chlorophenol 4-monooxygenase activity; 6-hydroxy-3-methyl-2-oxo-1,2-dihydroquinoline 6-monooxygenase activity; metal ion binding; oxidoreductase activity; carbon disulfide oxygenase activity; toluene 4-monooxygenase activity; phenanthrene 9,10-monooxygenase activity; 1-hydroxy-2-oxolimonene 1,2-monooxygenase activity; 2-hydroxy-phenylacetate hydroxylase activity; 4-chlorobenzaldehyde oxidase activity; enzyme binding; 2-oxo-delta3-4,5,5-trimethylcyclopentenylacetyl-CoA 1,2-monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; limonene monooxygenase activity; dibenzothiophene monooxygenase activity; 3,5-xylenol methylhydroxylase activity; phenanthrene 1,2-monooxygenase activity; 3-methyl-2-oxo-1,2-dihydroquinoline 6-monooxygenase activity; toluene 3-monooxygenase activity; styrene monooxygenase activity; monooxygenase activity; dimethyl sulfide monooxygenase activity; 4-hydroxyphenylacetate 3-monooxygenase activity; 4-nitrophenol 4-monooxygenase activity; 1-hydroxy-2-naphthoate hydroxylase activity; toluene-4-sulfonate monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; tetrahydrofuran hydroxylase activity; oxygen binding; phenylacetate hydroxylase activity; toluene 2-monooxygenase activity; iron ion binding; 6-hydroxynicotinate 3-monooxygenase activity; heme binding; arachidonic acid epoxygenase activity; phenanthrene 3,4-monooxygenase activity; alpha-pinene monooxygenase [NADH] activity; steroid hydroxylase activity; 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase
Biological Process: steroid metabolic process; triacylglycerol metabolic process; xenobiotic metabolic process; exogenous drug catabolic process; monoterpenoid metabolic process; epoxygenase P450 pathway; heterocycle metabolic process; drug metabolic process
Research Articles on CYP2E1
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Product Notes
The CYP2E1 cyp2e1 (Catalog #AAA176396) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cytochrome P450 2E1 antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome P450 2E1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Researchers should empirically determine the suitability of the CYP2E1 cyp2e1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome P450 2E1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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