Western Blot (WB)
(Western blot analysis of Cytochrome P450 26A1 expression in HepG2 (A), HEK293T (B) whole cell lysates.)
Rabbit Cytochrome P450 26A1 Polyclonal Antibody | anti-CYP26A1 antibody
Anti-Cytochrome P450 26A1 Antibody
IF/ICC (1/100 - 1/500)
IP (1/10 - 1/100)
Upon delivery aliquot and store at -20°C for one year.
Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of Cytochrome P450 26A1 expression in HepG2 (A), HEK293T (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Cytochrome P450 26A1 expression in HepG2 (A), HEK293T (B) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Cytochrome P450 26A1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of Cytochrome P450 26A1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein acts on retinoids, including all-trans-retinoic acid (RA), with both 4-hydroxylation and 18-hydroxylation activities. This enzyme regulates the cellular level of retinoic acid which is involved in regulation of gene expression in both embryonic and adult tissues. Two alternatively spliced transcript variants of this gene, which encode the distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]
Uniprot Description
CYP26A1: Plays a key role in retinoic acid metabolism. Acts on retinoids, including all-trans-retinoic acid (RA) and its stereoisomer 9-cis-RA. Capable of both 4-hydroxylation and 18- hydroxylation. Responsible for generation of several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA and 18-OH-RA. Belongs to the cytochrome P450 family.
Protein type: Cofactor and Vitamin Metabolism - retinol; Endoplasmic reticulum; EC 1.14.-.-; Oxidoreductase
Chromosomal Location of Human Ortholog: 10q23-q24
Cellular Component: endoplasmic reticulum membrane
Molecular Function: retinoic acid binding; iron ion binding; retinoic acid 4-hydroxylase activity; heme binding; oxygen binding
Biological Process: retinoic acid receptor signaling pathway; anterior/posterior pattern formation; negative regulation of retinoic acid receptor signaling pathway; vitamin metabolic process; central nervous system development; neural crest cell development; metabolic process; xenobiotic metabolic process
Research Articles on CYP26A1
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Product Notes
The CYP26A1 cyp26a1 (Catalog #AAA822084) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cytochrome P450 26A1 Antibody reacts with Human, Mouse, Rat, Bovine, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome P450 26A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemsitry (ICC), Immunoprecipitation (IP). WB (1/500 - 1/1000) IF/ICC (1/100 - 1/500) IP (1/10 - 1/100). Researchers should empirically determine the suitability of the CYP26A1 cyp26a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome P450 26A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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