Rabbit anti-Human Cysteine-rich, Angiogenic Inducer 61 Polyclonal Antibody | anti-CYR61 antibody

Cysteine-rich, Angiogenic Inducer 61 (CYR61)

Cat. Num. AAA614197

• Gene Names: CYR61; CCN1; GIG1; IGFBP10
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Cysteine-rich; Angiogenic Inducer 61; Polyclonal Antibody; Angiogenic Inducer 61 (CYR61); Anti -Cysteine-rich; anti-CYR61 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CYR61. Species sequence homology: rat and mouse 88%.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-CYR61 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunofluorescence, Immunocytochemistry and Immunohistochemistry.; Dilution: Immunohistochemistry (FFPE): 1:100; Western Blot (ECL): 1:200. A band can be seen at ~37kD representing the processed form of CYR61 protein.
• Immunogen: Synthetic peptide corresponding to the human CYR61 protein sequence (between residues 150-250).
• Positive Control: MDA-MB-231 breast cancer cell lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Detection of cleaved CYR61 in MDA-MB-231 cell lysate using MBS614197 Photo courtesy of Dr. Lupu's Lab, Northwestern University. Image by Dr. Ingrid Espinoza.)

Testing Data

(Human endometrium using MBS614197. Photo courtesy of Dr. rer. nat. Isabella Gashaw, Universität Duisburg-Essen)

Testing Data

Related Product Information for anti-CYR61 antibody

CYR61 is a cysteine-rich, heparin-binding, growth factor belonging to the CCN family of secreted regulatory proteins. It stimulates chondrogensis and angiogenesis, playing an important role in cartilage and bone formation, as well as tumor growth and vascularization.

Product Categories/Family for anti-CYR61 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 119593604
• NCBI GeneID: 3491
• UniProt Accession #: O00622; O14934; O43775; Q9BZL7; O95694; Q53FA4; Q6FI18; Q9UID7
• Molecular Weight: 42,027 Da
• NCBI Official Full Name: cysteine-rich, angiogenic inducer, 61, isoform CRA_a
• NCBI Official Synonym Full Names: cysteine-rich, angiogenic inducer, 61
• NCBI Official Symbol: CYR61
• NCBI Official Synonym Symbols: CCN1; GIG1; IGFBP10
• NCBI Protein Information: protein CYR61; IBP-10; IGFBP-10; OTTHUMP00000012303; CCN family member 1; IGF-binding protein 10; cysteine-rich, anigogenic inducer, 61; cysteine-rich heparin-binding protein 61; insulin-like growth factor-binding protein 10
• UniProt Protein Name: Protein CYR61
• Protein Family: Protein
• UniProt Gene Name: CYR61
• UniProt Synonym Gene Names: CCN1; GIG1; IGFBP10
• UniProt Entry Name: CYR61_HUMAN

NCBI Description

CYR61 is a secreted, cysteine-rich, heparin-binding protein encoded by a growth factor-inducible immediate-early gene. Acting as an extracellular, matrix-associated signaling molecule, CYR61 promotes the adhesion of endothelial cells through interaction with integrin and augments growth factor-induced DNA synthesis in the same cell type.[supplied by OMIM]

Uniprot Description

CYR61: Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up- regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. Belongs to the CCN family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p22.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix

Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding; extracellular matrix binding

Biological Process: extracellular matrix organization and biogenesis; anatomical structure morphogenesis; positive regulation of caspase activity; chemotaxis; intussusceptive angiogenesis; osteoblast differentiation; positive regulation of osteoblast differentiation; cell proliferation; cell-cell signaling; positive regulation of protein kinase activity; positive regulation of osteoblast proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of cell growth; positive regulation of protein amino acid phosphorylation; cell adhesion; positive regulation of BMP signaling pathway; negative regulation of apoptosis; positive regulation of cell migration