Rabbit anti-Human CYSLTR1 Polyclonal Antibody | anti-CYSLTR1 antibody

CYSLTR1, ID (CYSLTR1, CYSLT1, Cysteinyl leukotriene receptor 1, Cysteinyl leukotriene D4 receptor, G-protein coupled receptor HG55, HMTMF81) (FITC)

Cat. Num. AAA6290565

• Gene Names: CYSLTR1; HG55; CYSLT1; CYSLTR; CYSLT1R; HMTMF81
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CYSLTR1; Polyclonal Antibody; ID (CYSLTR1; CYSLT1; Cysteinyl leukotriene receptor 1; Cysteinyl leukotriene D4 receptor; G-protein coupled receptor HG55; HMTMF81) (FITC); HMTMF81; anti-CYSLTR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CYSLTR1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CYSLTR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-172 amino acids from the Central region of human CYSLTR1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CYSLTR1 Antibody (Center) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the CYSLTR1 antibody detected the CYSLTR1 protein (arrow).)

Immunohistochemistry (IHC)

(CYSLTR1 Antibody (Center) (AP14813c)immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYSLTR1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-CYSLTR1 antibody

The cysteinyl leukotrienes LTC4, LTD4, and LTE4 are important mediators of human bronchial asthma. Pharmacologic studies have determined that cysteinyl leukotrienes activate at least 2 receptors, the protein encoded by this gene and CYSLTR2. This encoded receptor is a member of the superfamily of G protein-coupled receptors. Activation of this receptor by LTD4 results in contraction and proliferation of smooth muscle, oedema, eosinophil migration and damage to the mucus layer in the lung.

Product Categories/Family for anti-CYSLTR1 antibody

Antibodies; G Protein Receptors

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Boulay, M.E., et al. Prostaglandins Leukot. Essent. Fatty Acids 83(1):15-22(2010). Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010). Hasegawa, S., et al. Platelets 21(4):253-259(2010). Sokolowska, M., et al. BMC Immunol. 10, 63 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 5729798
• NCBI GeneID: 10800
• NCBI Accession #: NP_006630.1
• NCBI GenBank Nucleotide #: NM_006639.2
• UniProt Accession #: Q9Y271; Q5JS94; Q8IV19; B2R954; D3DTE4
• Molecular Weight: 38,541 Da
• NCBI Official Full Name: cysteinyl leukotriene receptor 1
• NCBI Official Synonym Full Names: cysteinyl leukotriene receptor 1
• NCBI Official Symbol: CYSLTR1
• NCBI Official Synonym Symbols: HG55; CYSLT1; CYSLTR; CYSLT1R; HMTMF81
• NCBI Protein Information: cysteinyl leukotriene receptor 1; LTD4 receptor; G-protein coupled receptor HG55; cysteinyl leukotriene D4 receptor
• UniProt Protein Name: Cysteinyl leukotriene receptor 1
• Protein Family: Cysteinyl leukotriene receptor
• UniProt Gene Name: CYSLTR1
• UniProt Synonym Gene Names: CYSLT1; CysLTR1
• UniProt Entry Name: CLTR1_HUMAN

NCBI Description

The cysteinyl leukotrienes LTC4, LTD4, and LTE4 are important mediators of human bronchial asthma. Pharmacologic studies have determined that cysteinyl leukotrienes activate at least 2 receptors, the protein encoded by this gene and CYSLTR2. This encoded receptor is a member of the superfamily of G protein-coupled receptors. Activation of this receptor by LTD4 results in contraction and proliferation of smooth muscle, oedema, eosinophil migration and damage to the mucus layer in the lung. [provided by RefSeq, Jul 2008]

Uniprot Description

CYSLTR1: Receptor for cysteinyl leukotrienes mediating bronchoconstriction of individuals with and without asthma. Stimulation by LTD4 results in the contraction and proliferation of smooth muscle, edema, eosinophil migration and damage to the mucus layer in the lung. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTD4 >> LTE4 = LTC4 >> LTB4. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1

Chromosomal Location of Human Ortholog: Xq13.2-q21.1

Cellular Component: membrane; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; leukotriene receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; positive regulation of angiogenesis; calcium ion transport; positive regulation of vasoconstriction; defense response; respiratory gaseous exchange; inflammatory response; chemotaxis