Rabbit anti-Human CYSLTR1 Polyclonal Antibody | anti-CYSLTR1 antibody

CYSLTR1, ID (CYSLTR1, CYSLT1, Cysteinyl leukotriene receptor 1, Cysteinyl leukotriene D4 receptor, G-protein coupled receptor HG55, HMTMF81)

Cat. Num. AAA649090

• Gene Names: CYSLTR1; CYSLT1; CYSLTR; CYSLT1R; HMTMF81
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: CYSLTR1; Polyclonal Antibody; ID (CYSLTR1; CYSLT1; Cysteinyl leukotriene receptor 1; Cysteinyl leukotriene D4 receptor; G-protein coupled receptor HG55; HMTMF81); Anti -CYSLTR1; anti-CYSLTR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-CYSLTR1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50
• Immunogen: CYSLTR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-172 amino acids from the Central region of human CYSLTR1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CYSLTR1 Antibody (Center) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the CYSLTR1 antibody detected the CYSLTR1 protein (arrow).)

Immunohistochemistry (IHC)

(CYSLTR1 Antibody (Center) (AP14813c)immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYSLTR1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-CYSLTR1 antibody

The cysteinyl leukotrienes LTC4, LTD4, and LTE4 are important mediators of human bronchial asthma. Pharmacologic studies have determined that cysteinyl leukotrienes activate at least 2 receptors, the protein encoded by this gene and CYSLTR2. This encoded receptor is a member of the superfamily of G protein-coupled receptors. Activation of this receptor by LTD4 results in contraction and proliferation of smooth muscle, oedema, eosinophil migration and damage to the mucus layer in the lung.

Product Categories/Family for anti-CYSLTR1 antibody

Antibodies; Abs to G Protein Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 532164737
• NCBI GeneID: 10800
• NCBI Accession #: NP_001269117.1
• NCBI GenBank Nucleotide #: NM_001282188.1
• UniProt Accession #: Q9Y271; Q5JS94; Q8IV19; B2R954; D3DTE4
• Molecular Weight: 38,541 Da
• NCBI Official Full Name: cysteinyl leukotriene receptor 1
• NCBI Official Synonym Full Names: cysteinyl leukotriene receptor 1
• NCBI Official Symbol: CYSLTR1
• NCBI Official Synonym Symbols: CYSLT1; CYSLTR; CYSLT1R; HMTMF81
• NCBI Protein Information: cysteinyl leukotriene receptor 1; LTD4 receptor; G-protein coupled receptor HG55; cysteinyl leukotriene D4 receptor
• UniProt Protein Name: Cysteinyl leukotriene receptor 1
• Protein Family: Cysteinyl leukotriene receptor
• UniProt Gene Name: CYSLTR1
• UniProt Synonym Gene Names: CYSLT1; CysLTR1
• UniProt Entry Name: CLTR1_HUMAN

NCBI Description

This gene encodes a member of the G-protein coupled receptor 1 family. The encoded protein is a receptor for cysteinyl leukotrienes, and is involved in mediating bronchoconstriction via activation of a phosphatidylinositol-calcium second messenger system. Activation of the encoded receptor results in contraction and proliferation of bronchial smooth muscle cells, eosinophil migration, and damage to the mucus layer in the lung. Upregulation of this gene is associated with asthma and dysregulation may also be implicated in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

Function: Receptor for cysteinyl leukotrienes mediating bronchoconstriction of individuals with and without asthma. Stimulation by LTD4 results in the contraction and proliferation of smooth muscle, edema, eosinophil migration and damage to the mucus layer in the lung. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTD4 >> LTE4 = LTC4 >> LTB4.

Subcellular location: Cell membrane; Multi-pass membrane protein.

Tissue specificity: Widely expressed, with highest levels in spleen and peripheral blood leukocytes. Lower expression in several tissues, such as lung (mostly in smooth muscle bundles and alveolar macrophages), placenta, small intestine, pancreas, colon and heart.

Miscellaneous: Selective antagonists, such as montelukast (Singulair), zafirlukast (Accolate) and pranlukast (Onon), are used in the treatment of the asthma crisis.

Sequence similarities: Belongs to the G-protein coupled receptor 1 family.