Rabbit anti-Human CYP4V2 Polyclonal Antibody | anti-CYP4V2 antibody

CYP4V2, CT (CYP4V2, Cytochrome P450 4V2) (Biotin)

Cat. Num. AAA6290542

• Gene Names: CYP4V2; BCD; CYP4AH1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CYP4V2; Polyclonal Antibody; CT (CYP4V2; Cytochrome P450 4V2) (Biotin); Cytochrome P450 4V2; anti-CYP4V2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CYP4V2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CYP4V2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 495-525 amino acids from the C-terminal region of human CYP4V2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CYP4V2 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the CYP4V2 antibody detected the CYP4V2 protein (arrow).)

Related Product Information for anti-CYP4V2 antibody

This gene encodes a member of the cytochrome P450 hemethiolate protein superfamily which are involved in oxidizing various substrates in the metabolic pathway. It is implicated in the metabolism of fatty acid precursors into n-3 polyunsaturated fatty acids.

Product Categories/Family for anti-CYP4V2 antibody

Antibodies; Enzymes; Cytochrome

References

Yokoi, Y., et al. Acta Ophthalmol 88(5):607-609(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Pelak, K., et al. J. Infect. Dis. 201(8):1141-1149(2010). Nakano, M., et al. Drug Metab. Dispos. 37(11):2119-2122(2009). Li, Y., et al. J. Thromb. Haemost. 7(11):1802-1808(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 187960086
• NCBI GeneID: 285440
• NCBI Accession #: NP_997235.3
• NCBI GenBank Nucleotide #: NM_207352.3
• UniProt Accession #: Q6ZWL3; Q6ZTM4; B7U6W2
• Molecular Weight: 60,724 Da
• NCBI Official Full Name: cytochrome P450 4V2
• NCBI Official Synonym Full Names: cytochrome P450, family 4, subfamily V, polypeptide 2
• NCBI Official Symbol: CYP4V2
• NCBI Official Synonym Symbols: BCD; CYP4AH1
• NCBI Protein Information: cytochrome P450 4V2
• UniProt Protein Name: Cytochrome P450 4V2
• Protein Family: Cytochrome
• UniProt Gene Name: CYP4V2
• UniProt Entry Name: CP4V2_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 hemethiolate protein superfamily which are involved in oxidizing various substrates in the metabolic pathway. It is implicated in the metabolism of fatty acid precursors into n-3 polyunsaturated fatty acids. Mutations in this gene result in Bietti crystalline corneoretinal dystrophy. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP4V2: Catalyzes the omega-hydroxylation of medium-chain saturated fatty acids such as laurate, myristate and palmitate in an NADPH-dependent pathway. The substrate specificity is higher for myristate > laurate > palmitate (C14>C16>C12). May have a role in fatty acid and steroid metabolism in the eye. Defects in CYP4V2 are a cause of Bietti crystalline corneoretinal dystrophy (BCD). BCD is an autosomal recessive retinal dystrophy characterized by multiple glistening intraretinal crystals scattered over the fundus, a characteristic degeneration of the retina, and sclerosis of the choroidal vessels, ultimately resulting in progressive night blindness and constriction of the visual field. Most cases have similar crystals at the corneoscleral limbus. Clinically, BCD is progressive. Patients develop decreased vision, nyctalopia, and paracentral scotomata between the 2nd and 4th decade of life. Later, patients develop peripheral visual field loss and marked visual impairment, usually progressing to legal blindness by the 5th or 6th decade of life. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.14.13.199; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q35.2

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; iron ion binding; heme binding; monooxygenase activity

Biological Process: visual perception; response to stimulus; fatty acid omega-oxidation

Disease: Bietti Crystalline Corneoretinal Dystrophy