Rabbit anti-Human CYP3A5 Polyclonal Antibody | anti-CYP3A5 antibody

CYP3A5, ID (Cytochrome P450 3A5, CYPIIIA5, Cytochrome P450-PCN3, Cytochrome P450 HLp2)

Cat. Num. AAA624465

• Gene Names: CYP3A5; CP35; PCN3; CYPIIIA5; FLJ31317; P450PCN3; DKFZp686L16231
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: CYP3A5; Polyclonal Antibody; ID (Cytochrome P450 3A5; CYPIIIA5; Cytochrome P450-PCN3; Cytochrome P450 HLp2); Anti -CYP3A5; anti-CYP3A5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CYP3A5.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-CYP3A5 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100
• Immunogen: Synthetic peptide selected from the Center region of human CYP3A5 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CYP3A5 Antibody (Center) in 293 cell line lysates (35ug/lane). CYP3A5 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with GPD1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CYP3A5 Antibody (Center) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CYP3A5 antibody

CYP3A5 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. The enzyme metabolizes drugs such as nifedipine and cyclosporine as well as the steroid hormones testosterone, progesterone and androstenedione.

Product Categories/Family for anti-CYP3A5 antibody

Antibodies; Abs to Enzymes; Cytochrome

NCBI and Uniprot Product Information

• NCBI GI #: 306518609
• NCBI GeneID: 1577
• NCBI Accession #: NP_001177413.1
• NCBI GenBank Nucleotide #: NM_001190484.1
• UniProt Accession #: P20815; Q53WY8; Q75MV0; Q9HB56; A4D289; Q14V85; Q53GC3; Q7Z3N0; Q7Z446; Q7Z447; Q86SK1; Q96RK6; Q96RK8
• Molecular Weight: 57,109 Da
• NCBI Official Full Name: cytochrome P450 3A5 isoform 2
• NCBI Official Synonym Full Names: cytochrome P450, family 3, subfamily A, polypeptide 5
• NCBI Official Symbol: CYP3A5
• NCBI Official Synonym Symbols: CP35; PCN3; CYPIIIA5; FLJ31317; P450PCN3; DKFZp686L16231
• NCBI Protein Information: cytochrome P450 3A5; cytochrome P-450; OTTHUMP00000210469; OTTHUMP00000210470; niphedipine oxidase; cytochrome P450 HLp2; cytochrome P450-PCN3; microsomal monooxygenase; xenobiotic monooxygenase; aryl hydrocarbon hydroxylase; flavoprotein-linked monooxygenase; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 5
• UniProt Protein Name: Cytochrome P450 3A5
• UniProt Gene Name: CYP3A5
• UniProt Entry Name: CP3A5_HUMAN

NCBI Description

This gene,CYP3A5, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. The enzyme metabolizes drugs such as nifedipine and cyclosporine as well as the steroid hormones testosterone, progesterone and androstenedione. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. This cluster includes a pseudogene, CYP3A5P1, which is very similar to CYP3A5. This similarity has caused some difficulty in determining whether cloned sequences represent the gene or the pseudogene. Multiple alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq]

Uniprot Description

CYP3A5: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Xenobiotic Metabolism - metabolism by cytochrome P450; Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - drug metabolism - other enzymes; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Lipid Metabolism - linoleic acid; EC 1.14.14.1; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q21.1

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle

Molecular Function: iron ion binding; heme binding; oxidoreductase activity; oxygen binding; monooxygenase activity

Biological Process: steroid metabolic process; drug catabolic process; xenobiotic metabolic process; alkaloid catabolic process

Disease: Hypertension, Essential