Rabbit anti-Human CYP2B6 Polyclonal Antibody | anti-CYP2B6 antibody

CYP2B6, ID (CYP2B6, Cytochrome P450 2B6, CYPIIB6, Cytochrome P450 IIB1) (Biotin)

Cat. Num. AAA6290487

• Gene Names: CYP2B6; CPB6; EFVM; IIB1; P450; CYP2B; CYP2B7; CYP2B7P; CYPIIB6
• Reactivity: Human
• Applications: Immunofluorescence, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CYP2B6; Polyclonal Antibody; ID (CYP2B6; Cytochrome P450 2B6; CYPIIB6; Cytochrome P450 IIB1) (Biotin); Cytochrome P450 IIB1; anti-CYP2B6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CYP2B6 antibody: Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CYP2B6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-270 amino acids from the Central region of human CYP2B6.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CYP2B6 Antibody (Center) western blot analysis in 293(lane 1) and K562(lane 2) cell line lysates (35ug/lane).This demonstrates the CYP2B6 antibody detected the CYP2B6 protein (arrow).)

Immunohistochemistry (IHC)

(CYP2B6 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYP2B6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of CYP2B6 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-CYP2B6 antibody

This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q.

Product Categories/Family for anti-CYP2B6 antibody

Antibodies; Enzymes; Cytochrome

References

Figueroa, S.C., et al. Ther Drug Monit 32(5):579-585(2010). Chou, M., et al. Antimicrob. Agents Chemother. 54(10):4432-4439(2010). Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). Bunten, H., et al. Clin. Pharmacol. Ther. 88(3):383-389(2010). Gounden, V., et al. AIDS Res Ther 7, 32 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 7949031
• NCBI GeneID: 1555
• NCBI Accession #: NP_000758.1
• NCBI GenBank Nucleotide #: NM_000767.4
• UniProt Accession #: P20813; Q2V565; Q9UK46
• Molecular Weight: 56,278 Da
• NCBI Official Full Name: cytochrome P450 2B6
• NCBI Official Synonym Full Names: cytochrome P450, family 2, subfamily B, polypeptide 6
• NCBI Official Symbol: CYP2B6
• NCBI Official Synonym Symbols: CPB6; EFVM; IIB1; P450; CYP2B; CYP2B7; CYP2B7P; CYPIIB6
• NCBI Protein Information: cytochrome P450 2B6; cytochrome P450 IIB1; 1,4-cineole 2-exo-monooxygenase; cytochrome P450, subfamily IIB (phenobarbital-inducible), polypeptide 6
• UniProt Protein Name: Cytochrome P450 2B6
• Protein Family: Cytochrome
• UniProt Gene Name: CYP2B6
• UniProt Entry Name: CP2B6_HUMAN

NCBI Description

This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP2B6: cytochrome P450, subfamily IIB. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Phosphorylation is accompanied by a decrease in enzyme activity.

Protein type: Lipid Metabolism - arachidonic acid; Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - metabolism by cytochrome P450; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 1.14.14.1

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm

Molecular Function: iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; heme binding; arachidonic acid epoxygenase activity; steroid hydroxylase activity; oxygen binding; monooxygenase activity

Biological Process: steroid metabolic process; ketone metabolic process; xenobiotic metabolic process; exogenous drug catabolic process; epoxygenase P450 pathway; drug metabolic process

Disease: Efavirenz, Poor Metabolism Of