Rabbit BMAL1 Polyclonal Antibody | anti-BMAL1 antibody

BMAL1 Polyclonal Antibody

Cat. Num. AAA3012312

• Gene Names: ARNTL; TIC; JAP3; MOP3; BMAL1; PASD3; BMAL1c; bHLHe5
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: ProA affinity purified
• Synonyms: BMAL1; Polyclonal Antibody; BMAL1 Polyclonal Antibody; bHLHe5; ARNTL; BHLHE5; MOP3; PASD3; ARNT like protein 1 brain and muscle; Arntl; Aryl hydrocarbon receptor nuclear translocator like; Aryl hydrocarbon receptor nuclear translocator like protein 1; Aryl hydrocarbon receptor nuclear translocator-like protein 1; Basic helix loop helix PAS orphan MOP3; Basic helix loop helix PAS protein MOP3; Basic-helix-loop-helix-PAS protein MOP3; bHLH PAS protein JAP3; bHLH-PAS protein JAP3; BMAL 1; BMAL1c; Brain and muscle ARNT like 1; Brain and muscle ARNT-like 1; CG8727 PA; Class E basic helix-loop-helix protein 5; cycle; JAP 3; JAP3; Member of PAS protein 3; Member of PAS superfamily 3; MGC47515; MOP 3; PAS domain-containing protein 3; PASD 3; TIC; anti-BMAL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: BMAL1 polyclonal antibody detects endogenous levels of BMAL1 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 625

• Applicable Applications for anti-BMAL1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:1,000-1:2,000; ICC: 1:50-1:200
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of BMAL1 on different lysates using anti-BMAL1 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: Rat brain Lane 3: Mouse spleen Lane 4: Hela)

Immunocytochemistry (ICC)

(ICC staining BMAL1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-BMAL1 antibody

Transcriptional activator which forms a core component of the circadian clock. Transcription factors, CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes), harboring E-box elements (5'-CACGTG-3') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1/2 and RORA/B/G, which form a second feedback loop and which activate and repress ARNTL/BMAL1 transcription, respectively. ARNTL/BMAL1 positively regulates myogenesis and negatively regulates adipogenesis via the transcriptional control of the genes of the canonical Wnt signaling pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 71852580
• NCBI GeneID: 406
• NCBI Accession #: NP_001025443.1
• NCBI GenBank Nucleotide #: NP_001025443.1
• UniProt Accession #: O00327; Q9WTL8; Q9EPW1
• Molecular Weight: 68kDa
• NCBI Official Full Name: aryl hydrocarbon receptor nuclear translocator-like protein 1 isoform a
• NCBI Official Synonym Full Names: aryl hydrocarbon receptor nuclear translocator like
• NCBI Official Symbol: ARNTL
• NCBI Official Synonym Symbols: TIC; JAP3; MOP3; BMAL1; PASD3; BMAL1c; bHLHe5
• NCBI Protein Information: aryl hydrocarbon receptor nuclear translocator-like protein 1
• UniProt Protein Name: Aryl hydrocarbon receptor nuclear translocator-like protein 1
• UniProt Gene Name: ARNTL
• UniProt Synonym Gene Names: BHLHE5; BMAL1; MOP3; PASD3; bHLHe5
• UniProt Entry Name: BMAL1_HUMAN

NCBI Description

The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]