Rabbit CYP27A1 Polyclonal Antibody | anti-CYP27A1 antibody

CYP27A1 (E131) Polyclonal Antibody

Cat. Num. AAA3002265

• Gene Names: CYP27A1; CTX; CP27; CYP27
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CYP27A1; Polyclonal Antibody; CYP27A1 (E131) Polyclonal Antibody; Sterol 26-hydroxylase; mitochondrial; 5-beta-cholestane-3-alpha; 7-alpha; 12-alpha-triol 27-hydroxylase; Cytochrome P-450C27/25; Cytochrome P450 27; Sterol 27-hydroxylase; Vitamin D(3) 25-hydroxylase; CYP27; anti-CYP27A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CYP27A1 (E131) polyclonal antibody detects endogenous levels of CYP27A1 protein
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 531

• Applicable Applications for anti-CYP27A1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human CYP27A1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CYP27A1 (E131) pAb at 1:500 dilutionLane1:LO2 whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of CYP27A1 (E131) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-CYP27A1 antibody

CYP27A1 participates in the degradation of cholesterol to bile acids in both the classic and acidic pathways.[1] It is the initiating enzyme in the acidic pathway to bile acids, yielding oxysterols by introducing a hydroxyl group to the carbon at the 27 position in cholesterol. In the acidic pathway, it produces 27-hydroxycholesterol from cholesterol whereas in the classic or neutral pathway, it produces 3beta-hydroxy-5-cholestenoic acid.It is also involved in the metabolism of vitamin D3. While CYP27A1 is present in many different tissues, its function in these tissues is largely uncharacterized. In macrophages, 27-hydroxycholesterol generated by this enzyme may be helpful against the production of inflammatory factors associated with cardiovascular disease.

NCBI and Uniprot Product Information

• NCBI GI #: 4503211
• NCBI GeneID: 1593
• NCBI Accession #: NP_000775.1
• NCBI GenBank Nucleotide #: NP_000775.1
• UniProt Accession #: Q02318
• Molecular Weight: ~ 60kDa
• NCBI Official Full Name: sterol 26-hydroxylase, mitochondrial
• NCBI Official Synonym Full Names: cytochrome P450 family 27 subfamily A member 1
• NCBI Official Symbol: CYP27A1
• NCBI Official Synonym Symbols: CTX; CP27; CYP27
• NCBI Protein Information: sterol 26-hydroxylase, mitochondrial
• UniProt Protein Name: Sterol 26-hydroxylase, mitochondrial
• Protein Family: Sterol 26-hydroxylase
• UniProt Gene Name: CYP27A1
• UniProt Synonym Gene Names: CYP27
• UniProt Entry Name: CP27A_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP27A1: Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta- cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3- 25-hydroxylase activity. Defects in CYP27A1 are the cause of cerebrotendinous xanthomatosis (CTX). CTX is a rare sterol storage disorder characterized clinically by progressive neurologic dysfunction, premature atherosclerosis, and cataracts. Belongs to the cytochrome P450 family.

Protein type: Oxidoreductase; Lipid Metabolism - primary bile acid biosynthesis; EC 1.14.13.15; Mitochondrial

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: mitochondrial matrix; mitochondrial inner membrane

Molecular Function: cholestanetriol 26-monooxygenase activity; iron ion binding; cholesterol 26-hydroxylase activity; heme binding; steroid hydroxylase activity; vitamin D3 25-hydroxylase activity

Biological Process: bile acid biosynthetic process; cholesterol metabolic process; bile acid metabolic process; xenobiotic metabolic process; sterol metabolic process

Disease: Cerebrotendinous Xanthomatosis