Rabbit anti-Human, Mouse CYP26A1 Polyclonal Antibody | anti-CYP26A1 antibody

CYP26A1, CT (CYP26A1, CYP26, P450RAI1, Cytochrome P450 26A1, Cytochrome P450 retinoic acid-inactivating 1, Retinoic acid 4-hydroxylase, Retinoic acid-metabolizing cytochrome) (MaxLight 490)

Cat. Num. AAA6290458

• Gene Names: CYP26A1; CP26; CYP26; P450RAI; P450RAI1
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: CYP26A1; Polyclonal Antibody; CT (CYP26A1; CYP26; P450RAI1; Cytochrome P450 26A1; Cytochrome P450 retinoic acid-inactivating 1; Retinoic acid 4-hydroxylase; Retinoic acid-metabolizing cytochrome) (MaxLight 490); Retinoic acid-metabolizing cytochrome; anti-CYP26A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-CYP26A1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CYP26A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-506 amino acids from the C-terminal region of human CYP26A1.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CYP26A1 Antibody (C-term) in mouse NIH-3T3 cell lysates (35ug/lane).CYP26A1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(CYP26A1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CYP26A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CYP26A1 Antibody (C-term) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CYP26A1 antibody

CYP26A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids.This endoplasmic reticulum protein acts on retinoids, including all-trans-retinoic acid (RA), with both 4-hydroxylation and 18-hydroxylation activities. This enzyme regulates the cellular level of retinoic acid which is involved in regulation of gene expression in both embryonic and adult tissues.

Product Categories/Family for anti-CYP26A1 antibody

Antibodies; Enzymes; Cytochrome

References

Quere, R., Blood 109 (10), 4450-4460 (2007). Lee, S.J., Pharmacogenet. Genomics 17 (3), 169-180 (2007). Heise, R., J. Invest. Dermatol. 126 (11), 2473-2480 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 74747535
• NCBI GeneID: 1592
• UniProt Accession #: Q5VXI0; O43174
• NCBI Official Synonym Full Names: cytochrome P450 family 26 subfamily A member 1
• NCBI Official Symbol: CYP26A1
• NCBI Official Synonym Symbols: CP26; CYP26; P450RAI; P450RAI1
• NCBI Protein Information: cytochrome P450 26A1
• Protein Family: Cytochrome

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein acts on retinoids, including all-trans-retinoic acid (RA), with both 4-hydroxylation and 18-hydroxylation activities. This enzyme regulates the cellular level of retinoic acid which is involved in regulation of gene expression in both embryonic and adult tissues. Two alternatively spliced transcript variants of this gene, which encode the distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]