Rabbit anti-Human CYP1A1 Polyclonal Antibody | anti-CYP1A1 antibody

Anti-Human CYP1A1 DyLight 550 conjugated Antibody

Cat. Num. AAA1751248

• Gene Names: CYP1A1; AHH; AHRR; CP11; CYP1; CYPIA1; P1-450; P450-C; P450DX
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Immunogen Affinity Purified
• Synonyms: CYP1A1; Polyclonal Antibody; Anti-Human CYP1A1 DyLight 550 conjugated Antibody; Rabbit IgG Polyclonal Anti-Human CYP1A1 Antibody DyLight 550 Conjugated; Flow Validated; Cytochrome P450 1A1; CYPIA1; Cytochrome P450 form 6; Cytochrome P450-C; Cytochrome P450-P1; Cytochrome P450; family 1; subfamily A; polypeptide 1; anti-CYP1A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 189

• Applicable Applications for anti-CYP1A1 antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: 1-3ug/1x106 cells
• Immunogen: E Coli-derived human CYP1A1 recombinant protein (Position: H183-D320). Human CYP1A1 shares 81.2% amino acid (aa) sequence identity with both mouse and rat CYP1A1.
• Preparation and Storage: Store at 2-8 degree C for one year. Protect from light. Do not freeze.

Related Product Information for anti-CYP1A1 antibody

CYP1A1 is involved in phase I xenobiotic and drug metabolism (one substrate of it is theophylline). It is inhibited by fluoroquinolones and macrolides and induced by aromatic hydrocarbons. CYP1A1 is also known as AHH (aryl hydrocarbon hydroxylase). It is involved in the metabolic activation of aromatic hydrocarbons (polycyclic aromatic hydrocarbons, PAH), for example, benzo (a)pyrene (BP), by transforming it to an epoxide. In this reaction, the oxidation of benzo[a]pyrene is catalysed by CYP1A1 to form BP-7, 8-epoxide, which can be further oxidized by epoxide hydrolase (EH) to form BP-7, 8-dihydrodiol. Finally CYP1A1 catalyses this intermediate to form BP-7, 8-dihydrodiol-9, 10-epoxide, which is the ultimate carcinogen. However, an in vivo experiment with gene-deficient mice has found that the hydroxylation of benzo (a)pyrene by CYP1A1 can have an overall protective effect on the DNA, rather than contributing to potentially carcinogenic DNA modifications. This effect is likely due to the fact that CYP1A1 is highly active in the intestinal mucosa, and thus inhibits infiltration of ingested benzo (a)pyrene carcinogen into the systemic circulation.

References

1. Beresford AP (1993). "CYP1A1: friend or foe". Drug Metabolism Reviews 25 (4): 503-17. 2. Uno, S (2004). "Oral exposure to benzo[a]pyrene in the mouse: detoxication by inducible cytochrome P450 is more important than metabolic activation.". Mol Pharmacol 65 (5): 1225-37.

NCBI and Uniprot Product Information

• NCBI GI #: 4503199
• NCBI GeneID: 1543
• NCBI Accession #: NP_000490.1
• NCBI GenBank Nucleotide #: NM_000499.5
• UniProt Accession #: P04798; Q53G18; A4F3V9; A4F3W0
• Molecular Weight: 20,788 Da
• NCBI Official Full Name: cytochrome P450 1A1 isoform 1
• NCBI Official Synonym Full Names: cytochrome P450 family 1 subfamily A member 1
• NCBI Official Symbol: CYP1A1
• NCBI Official Synonym Symbols: AHH; AHRR; CP11; CYP1; CYPIA1; P1-450; P450-C; P450DX
• NCBI Protein Information: cytochrome P450 1A1
• UniProt Protein Name: Cytochrome P450 1A1
• Protein Family: Cytochrome
• UniProt Gene Name: CYP1A1

NCBI Description

This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2016]

Uniprot Description

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.