Immunofluorescence (IF)
(Confocal immunofluorescent analysis of CYP17A1 Antibody (Center) with Hela cell followed by Alexa Fluor??488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Rabbit CYP17A1 Polyclonal Antibody | anti-CYP17A1 antibody
CYP17A1 Antibody (Center)
WB~~1:1000
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of CYP17A1 Antibody (Center) with Hela cell followed by Alexa Fluor??488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of CYP17A1 Antibody (Center) with Hela cell followed by Alexa Fluor??488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Western Blot (WB)
(Western blot analysis of anti-CYP17A1 Antibody (Center) in K562 cell line lysates (35ug/lane). CYP17A1(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-CYP17A1 Antibody (Center) in K562 cell line lysates (35ug/lane). CYP17A1(arrow) was detected using the purified Pab.)
Nelson,D.R., Pharmacogenetics 14 (1), 1-18 (2004)
Imai,T., Hum. Genet. 89 (1), 95-96 (1992)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq, Jul 2008]
Uniprot Description
CYP17A1: Conversion of pregnenolone and progesterone to their 17- alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty. Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5). AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: salt wasting (SW, the most severe type), simple virilizing (SV, less severely affected patients), with normal aldosterone biosynthesis, non-classic form or late onset (NC or LOAH), and cryptic (asymptomatic). Belongs to the cytochrome P450 family.
Protein type: Oxidoreductase; EC 4.1.2.30; Lipid Metabolism - C21-steroid hormone; EC 1.14.99.9
Chromosomal Location of Human Ortholog: 10q24.3
Cellular Component: endoplasmic reticulum membrane; mitochondrion; cell soma; endoplasmic reticulum; axon
Molecular Function: 17-alpha-hydroxyprogesterone aldolase activity; steroid 17-alpha-monooxygenase activity; iron ion binding; heme binding; oxygen binding
Biological Process: steroid metabolic process; response to cAMP; dibenzo-p-dioxin metabolic process; androgen biosynthetic process; progesterone metabolic process; response to insecticide; Leydig cell differentiation; biphenyl metabolic process; response to methylmercury; hormone biosynthetic process; response to nutrient levels; response to drug; response to retinoic acid; adrenal gland development; phthalate metabolic process; hippocampus development; response to herbicide; ovulation; response to acetate; response to steroid hormone stimulus; response to cytokine stimulus; xenobiotic metabolic process; response to ionizing radiation; glucocorticoid biosynthetic process; sex differentiation; steroid biosynthetic process; sterol metabolic process; phenol metabolic process
Disease: Adrenal Hyperplasia, Congenital, Due To 17-alpha-hydroxylase Deficiency
Research Articles on CYP17A1
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Product Notes
The CYP17A1 cyp17a1 (Catalog #AAA9213005) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 358-388. The CYP17A1 Antibody (Center) reacts with Human (Predicted Reactivity: Monkey) and may cross-react with other species as described in the data sheet. AAA Biotech's CYP17A1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Western Blot (WB). IF~~1:10~50 WB~~1:1000. Researchers should empirically determine the suitability of the CYP17A1 cyp17a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYP17A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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