Rabbit anti-Human CYP11B1 Polyclonal Antibody | anti-CYP11B1 antibody

Anti-CYP11B1 antibody

Cat. Num. AAA175139

• Gene Names: CYP11B1; FHI; CPN1; CYP11B; P450C11
• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: CYP11B1; Polyclonal Antibody; Anti-CYP11B1 antibody; anti-CYP11B1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human.; No cross reactivity with other proteins
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 503

• Applicable Applications for anti-CYP11B1 antibody: Western Blot (WB)
• Application Notes: Western blot; Concentration: 0.1-0.5 ug/ml; Tested Species: Human; ; Other applications have not been tested.; Optimal dilutions should be determined by end user.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CYP11B1 (436-450aa RNFYHVPFGFGMRQC).
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg Thimerosal, 0.05mg NaN₃.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-CYP11B1 antibody, MBS175139, Western blottingAll lanes: Anti CYP11B1 (MBS175139) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: U87 Whole Cell Lysate at 40ugLane 3: MM231 Whole Cell Lysate at 40ugLane 4: PANC Whole Cell Lysate at 40ugLane 5: MM453 Whole Cell Lysate at 40ugLane 6: HELA Whole Cell Lysate at 40ugLane 7: SMMC Whole Cell Lysate at 40ugPredicted bind size: 58KDObserved bind size: 58KD)

Related Product Information for anti-CYP11B1 antibody

CYP11B1(Cytochrome p450, family 11, subfamily B, polypeptide 1), also called Steroid 11-beta-hydroxylase or P450C11, is a steroid hydroxylase found in the zona fasciculata. The CYP11B1 gene functions primarily in mitochondria in the zona fasciculata of the adrenal cortex to convert 11-deoxycortisol to cortisol and 11-deoxycorticosterone to corticosterone. CYP11B1 is a member of the cytochrome P450 superfamily of enzymes. It is mapped on 8q24.3. The CYP11B1 gene contains 9 exons and spans 6.5 kb. Using RT-PCR, Kayes-Wandover and White detected CYP11B1 mRNA in human cardiac tissue samples from left and right atria, aorta, apex, intraventricular septum, and atrioventricular node, as well as whole adult and fetal heart. Ventricles did not express CYP11B1. In patients with 11-beta-hydroxylase deficiency leading to hypertension and congenital adrenal hyperplasia, Pascoe et al. identified mutations in the CYP11B1 gene.

NCBI and Uniprot Product Information

• NCBI GI #: 215274267
• NCBI GeneID: 1584
• UniProt Accession #: P15538; Q14095; Q4VAQ8; Q4VAQ9; Q9UML2
• NCBI Official Full Name: Cytochrome P450 11B1, mitochondrial
• NCBI Official Synonym Full Names: cytochrome P450, family 11, subfamily B, polypeptide 1
• NCBI Official Symbol: CYP11B1
• NCBI Official Synonym Symbols: FHI; CPN1; CYP11B; P450C11
• NCBI Protein Information: cytochrome P450 11B1, mitochondrial; CYPXIB1; cytochrome P450C11; cytochrome P-450c11; cytochrome p450 XIB1; steroid 11-beta-hydroxylase; steroid 11-beta-monooxygenase; cytochrome P450, subfamily XIB (steroid 11-beta-hydroxylase), polypeptide 1
• UniProt Protein Name: Cytochrome P450 11B1, mitochondrial
• Protein Family: Cytochrome
• UniProt Gene Name: CYP11B1
• UniProt Synonym Gene Names: S11BH; Cytochrome P450C11
• UniProt Entry Name: C11B1_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and is involved in the conversion of progesterone to cortisol in the adrenal cortex. Mutations in this gene cause congenital adrenal hyperplasia due to 11-beta-hydroxylase deficiency. Transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP11B1: Has steroid 11-beta-hydroxylase activity. In addition to this activity, the 18 or 19-hydroxylation of steroids and the aromatization of androstendione to estrone have also been ascribed to cytochrome P450 XIB. Defects in CYP11B1 are the cause of adrenal hyperplasia type 4 (AH4). AH4 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: salt wasting (SW, the most severe type), simple virilizing (SV, less severely affected patients), with normal aldosterone biosynthesis, non-classic form or late onset (NC or LOAH), and cryptic (asymptomatic). AH4 patients usually have hypertension. Defects in CYP11B1 are a cause of familial hyperaldosteronism type 1 (FH1). It is a disorder characterized by hypertension, variable hyperaldosteronism, and abnormal adrenal steroid production, including 18-oxocortisol and 18-hydroxycortisol. There is significant phenotypic heterogeneity, and some individuals never develop hypertension. The molecular defect causing hyperaldosteronism familial type 1 is an anti-Lepore-type fusion of the CYP11B1 and CYP11B2 genes. The hybrid gene has the promoting part of CYP11B1, ACTH-sensitive, and the coding part of CYP11B2. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.14.15.4; Lipid Metabolism - C21-steroid hormone; Mitochondrial; Lipid Metabolism - androgen and estrogen

Chromosomal Location of Human Ortholog: 8q21

Cellular Component: mitochondrion; mitochondrial inner membrane

Molecular Function: steroid 11-beta-monooxygenase activity; iron ion binding; heme binding

Biological Process: steroid metabolic process; regulation of blood pressure; xenobiotic metabolic process; C21-steroid hormone biosynthetic process; immune response; glucocorticoid biosynthetic process; glucose homeostasis; sterol metabolic process; aldosterone biosynthetic process; cellular response to hormone stimulus

Disease: Glucocorticoid-remediable Aldosteronism; Adrenal Hyperplasia, Congenital, Due To Steroid 11-beta-hydroxylase Deficiency