Western Blot (WB)
(Western blot analysis of CYP11A1 expression in rat brain extract (lane 1), rat kidney extract (lane 2) and 293T whole cell lysates (lane 3). CYP11A1 at 75KD was detected using rabbit anti-CYP11A1 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Rabbit CYP11A1 Polyclonal Antibody | anti-CYP11A1 antibody
Anti-CYP11A1 Antibody
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Tested Species:In-house tested species with positive results.
Other applications have not been tested.
Western Blot (WB)
(Western blot analysis of CYP11A1 expression in rat brain extract (lane 1), rat kidney extract (lane 2) and 293T whole cell lysates (lane 3). CYP11A1 at 75KD was detected using rabbit anti-CYP11A1 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Western Blot (WB)
(Western blot analysis of CYP11A1 expression in rat brain extract (lane 1), rat kidney extract (lane 2) and 293T whole cell lysates (lane 3). CYP11A1 at 75KD was detected using rabbit anti-CYP11A1 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Background: CYP11A1 (Cytochrome p450, family 11, subfamily A, polypeptide 1), commonly referred to as P450scc, is a mitochondrial enzyme associated with the conversion of cholesterol to pregnenolone. CYP11A1 is a member of the cytochrome P450 superfamily of enzymes. The CYP11A1 gene is mapped on 15q24.1. Expression of the CYP11A1 gene may play a role in skin physiology and pathology and that cutaneous proopiomelanocortin activity may be autoregulated by a feedback mechanism involving glucocorticoids synthesized locally. Using in vitro studies, CYP11A1 catalyzed the side-chain cleavage of 7-dehydrocholesterol to form 7-dehydropregnenolone. In addition, CYP11A1 catalyzed the metabolism of biologically inert vitamin D3, which is formed from 7-dehydrocholesterol, to form 2 hydroxylated products, 20-hydroxyvitamin D3 and 20, 22-dihydroxyvitamin D3. Mutations in the CYP11A1 gene cause congenital adrenal insufficiency with partial or complete 46, XY sex reversal.
2. Chung, B.-C., Matteson, K. J., Voutilainen, R., Mohandas, T. K., Miller, W. L. Human cholesterol side-chain cleavage enzyme, P450scc: cDNA cloning, assignment of the gene to chromosome 15, and expression in the placenta. Proc. Nat. Acad. Sci. 83: 8962-8966, 1986.
3. Guryev, O., Carvalho, R. A., Usanov, S., Gilep, A., Estabrook, R. W. A pathway for the metabolism of vitamin D3: unique hydroxylated metabolites formed during catalysis with cytochrome P450scc (CYP11A1). Proc. Nat. Acad. Sci. 100: 14754-14759, 2003.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008]
Uniprot Description
CYP11A1: Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone. Defects in CYP11A1 are the cause of adrenal insufficiency congenital with 46,XY sex reversal (AICSR). A rare disorder that can present as acute adrenal insufficiency in infancy or childhood. ACTH and plasma renin activity are elevated and adrenal steroids are inappropriately low or absent; the 46,XY patients have female external genitalia, sometimes with clitoromegaly. The phenotypic spectrum ranges from prematurity, complete underandrogenization, and severe early-onset adrenal failure to term birth with clitoromegaly and later-onset adrenal failure. Patients with congenital adrenal insufficiency do not manifest the massive adrenal enlargement typical of congenital lipoid adrenal hyperplasia. Belongs to the cytochrome P450 family.
Protein type: EC 1.14.15.6; Oxidoreductase; Mitochondrial; Lipid Metabolism - C21-steroid hormone
Chromosomal Location of Human Ortholog: 15q23-q24
Cellular Component: mitochondrial inner membrane; mitochondrial matrix; mitochondrion
Molecular Function: cholesterol monooxygenase (side-chain-cleaving) activity; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen
Biological Process: C21-steroid hormone biosynthetic process; cholesterol metabolic process; glucocorticoid biosynthetic process; sterol metabolic process; vitamin D metabolic process
Disease: Adrenal Insufficiency, Congenital, With 46,xy Sex Reversal, Partial Or Complete
Research Articles on CYP11A1
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Product Notes
The CYP11A1 cyp11a1 (Catalog #AAA178442) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CYP11A1 Antibody reacts with Human, Rat No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's CYP11A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: 0.1-0.5mug/ml; Tested Species: Human, Rat Tested Species:In-house tested species with positive results. Other applications have not been tested. Researchers should empirically determine the suitability of the CYP11A1 cyp11a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYP11A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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