Rabbit APEH Polyclonal Antibody | anti-APEH antibody

APEH Polyclonal Antibody

Cat. Num. AAA9125195

• Gene Names: APEH; APH; OPH; AARE; ACPH; D3S48E; D3F15S2; DNF15S2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: APEH; Polyclonal Antibody; APEH Polyclonal Antibody; APH; OPH; AARE; ACPH; D3S48E; D3F15S2; DNF15S2; anti-APEH antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 732

• Applicable Applications for anti-APEH antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human APEH
• Calculated Molecular Weight: 81kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using APEH antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of 100ug extracts of SW480 cells using 3ug APEH antibody. Western blot was performed from the immunoprecipitate using APEH antibody at a dilition of 1:1000.)

Related Product Information for anti-APEH antibody

This gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma.

Product Categories/Family for anti-APEH antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 38258902
• NCBI GeneID: 327
• UniProt Accession #: P13798; Q9BQ33; Q9P0Y2
• Molecular Weight: 732
• NCBI Official Full Name: Acylamino-acid-releasing enzyme
• NCBI Official Synonym Full Names: acylaminoacyl-peptide hydrolase
• NCBI Official Symbol: APEH
• NCBI Official Synonym Symbols: APH; OPH; AARE; ACPH; D3S48E; D3F15S2; DNF15S2
• NCBI Protein Information: acylamino-acid-releasing enzyme; acyl-peptide hydrolase; acylaminoacyl-peptidase; oxidized protein hydrolase; N-acylaminoacyl-peptide hydrolase
• UniProt Protein Name: Acylamino-acid-releasing enzyme
• Protein Family: Acylamino-acid-releasing enzyme
• UniProt Gene Name: APEH
• UniProt Synonym Gene Names: D3F15S2; D3S48E; DNF15S2; AARE; APH; OPH
• UniProt Entry Name: ACPH_HUMAN

NCBI Description

This gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma. [provided by RefSeq, Jul 2008]

Uniprot Description

APEH: This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N- acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser. Belongs to the peptidase S9C family.

Protein type: Protease; EC 3.4.19.1

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: nuclear membrane; cytoplasm

Molecular Function: omega peptidase activity; serine-type endopeptidase activity

Biological Process: beta-amyloid metabolic process; proteolysis