Human Cholesterol side-chain cleavage enzyme, mitochondrial ELISA Kit | CYP11A1 elisa kit

Human Cholesterol side-chain cleavage enzyme, mitochondrial ELISA Kit

Cat. Num. AAA763017

• Gene Names: CYP11A1; CYP11A; CYPXIA1; P450SCC
• Reactivity: Human
• Synonyms: Cholesterol side-chain cleavage enzyme; mitochondrial; Human Cholesterol side-chain cleavage enzyme; mitochondrial ELISA Kit; CYP11A1 (Cholesterol side-chain cleavage enzyme; mitochondrial)/CYP11A/Cytochrome P450 (scc)/Cytochrome P450 11A1/Cholesterol desmolase/; CYP11A1 elisa kit

• Reactivity: Human
• Sequence Length: 521

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CYP11A1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human CYP11A1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-human CYP11A1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CYP11A1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofhuman CYP11A1can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 153218646
• NCBI GeneID: 1583
• NCBI Accession #: NP_000772.2
• NCBI GenBank Nucleotide #: NM_000781.2
• Molecular Weight: 42,152 Da
• NCBI Official Full Name: cholesterol side-chain cleavage enzyme, mitochondrial isoform a
• NCBI Official Synonym Full Names: cytochrome P450 family 11 subfamily A member 1
• NCBI Official Symbol: CYP11A1
• NCBI Official Synonym Symbols: CYP11A; CYPXIA1; P450SCC
• NCBI Protein Information: cholesterol side-chain cleavage enzyme, mitochondrial
• UniProt Protein Name: Cholesterol side-chain cleavage enzyme, mitochondrial
• Protein Family: Cholesterol side-chain cleavage enzyme
• UniProt Gene Name: CYP11A1
• UniProt Synonym Gene Names: CYP11A
• UniProt Entry Name: CP11A_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP11A1: Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone. Defects in CYP11A1 are the cause of adrenal insufficiency congenital with 46,XY sex reversal (AICSR). A rare disorder that can present as acute adrenal insufficiency in infancy or childhood. ACTH and plasma renin activity are elevated and adrenal steroids are inappropriately low or absent; the 46,XY patients have female external genitalia, sometimes with clitoromegaly. The phenotypic spectrum ranges from prematurity, complete underandrogenization, and severe early-onset adrenal failure to term birth with clitoromegaly and later-onset adrenal failure. Patients with congenital adrenal insufficiency do not manifest the massive adrenal enlargement typical of congenital lipoid adrenal hyperplasia. Belongs to the cytochrome P450 family.

Protein type: Lipid Metabolism - C21-steroid hormone; Mitochondrial; EC 1.14.15.6; Oxidoreductase

Chromosomal Location of Human Ortholog: 15q23-q24

Cellular Component: mitochondrial inner membrane; mitochondrial matrix; mitochondrion

Molecular Function: cholesterol monooxygenase (side-chain-cleaving) activity; heme binding; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen

Biological Process: C21-steroid hormone biosynthetic process; cholesterol metabolic process; glucocorticoid biosynthetic process; steroid metabolic process; sterol metabolic process; vitamin D metabolic process; xenobiotic metabolic process

Disease: Adrenal Insufficiency, Congenital, With 46,xy Sex Reversal, Partial Or Complete