Western Blot (WB)
(Western blot detection of Cyclophilin F in K562,3T3,Hela cell lysates using Cyclophilin F Rabbit pAb(1:1000 diluted). Predicted band size:22kDa. Observed band size:18kDa.)
Rabbit Cyclophilin F Polyclonal Antibody | anti-PPIF antibody
Cyclophilin F Rabbit pAb
IHC: 1:500
ICC/IF: 1:20
FC/FACS: 1:100
Western Blot (WB)
(Western blot detection of Cyclophilin F in K562,3T3,Hela cell lysates using Cyclophilin F Rabbit pAb(1:1000 diluted). Predicted band size:22kDa. Observed band size:18kDa.)
Western Blot (WB)
(Western blot detection of Cyclophilin F in K562,3T3,Hela cell lysates using Cyclophilin F Rabbit pAb(1:1000 diluted). Predicted band size:22kDa. Observed band size:18kDa.)
PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:20676357). Involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated. In cooperation with mitochondrial TP53 is involved in activating oxidative stress-induced necrosis. Involved in modulation of mitochondrial membrane F1F0 ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels. Has anti-apoptotic activity independently of mPTP and in cooperation with BCL2 inhibits cytochrome c-dependent apoptosis.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq, Jul 2008]
Uniprot Description
PPIF: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Belongs to the cyclophilin-type PPIase family.
Protein type: EC 5.2.1.8; Mitochondrial; Isomerase
Chromosomal Location of Human Ortholog: 10q22.3
Cellular Component: membrane; mitochondrial matrix; mitochondrial proton-transporting ATP synthase complex
Molecular Function: protein binding; peptidyl-prolyl cis-trans isomerase activity; cyclosporin A binding
Biological Process: apoptotic mitochondrial changes; protein peptidyl-prolyl isomerization; protein folding; regulation of mitochondrial membrane permeability; negative regulation of ATPase activity; negative regulation of apoptosis
Research Articles on PPIF
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Product Notes
The PPIF ppif (Catalog #AAA4751902) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cyclophilin F Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclophilin F can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB: 1:1000 IHC: 1:500 ICC/IF: 1:20 FC/FACS: 1:100. Researchers should empirically determine the suitability of the PPIF ppif for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclophilin F, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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