Fibroblast Growth Factor Receptor 1 Recombinant Protein | FGFR1 recombinant protein

Recombinant Fibroblast Growth Factor Receptor 1 (FGFR1)

Cat. Num. AAA2031824

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Fibroblast Growth Factor Receptor 1; Recombinant Fibroblast Growth Factor Receptor 1 (FGFR1); FGFR1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; RPAPTLPEQ AQPWGVPVEV ESLLVHPGDL LQLRCRLRDDVQSINWLRDG VQLAESNRTR ITGEEVEVRD SIPADSGLYA CVTNSPSGSD TTYFSVNVSDALPSSEDDDD DDDSSSEEKE TDNTKPNRRP VAPYWTSPEK MEKKLHAVPA AKTVKFKCPSSGTPSPTLRW LKNGKEFKPD HRIGGYKVRY ATWSIIMDSV VPSDKGNYTC IVENEYGSINHTYQLDVVER SPHRPILQAG LPANKTVALG SNVEFMCKVY SDPQPHIQWL KHIEVNGSKIGPDNLPYDQI LKTAGVNTTD KEMEVLHLRN VSFEDAGEYT CLAGNSIGLS HHSAWLTVLEALEERPAVMT SPLYLE
• Sequence Length: 822

• Applicable Applications for FGFR1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 43.2kDa
• Accurate Molecular Mass (KD): 48kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Rattus norvegicus (Rat)
• Fragment: Arg22~Glu376
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 13162333
• NCBI GeneID: 79114
• NCBI Accession #: NP_077060.1
• NCBI GenBank Nucleotide #: NM_024146.1
• Molecular Weight: 91,825 Da
• NCBI Official Full Name: fibroblast growth factor receptor 1
• NCBI Official Synonym Full Names: Fibroblast growth factor receptor 1
• NCBI Official Symbol: Fgfr1
• NCBI Protein Information: fibroblast growth factor receptor 1
• UniProt Protein Name: Fibroblast growth factor receptor 1
• Protein Family: Fibroblast growth factor receptor
• UniProt Gene Name: Fgfr1
• UniProt Synonym Gene Names: Flg; FGFR-1; bFGF-R-1
• UniProt Entry Name: FGFR1_RAT

NCBI Description

proportion of transmembrane and soluble extracellular isoforms may modulate fibroblast growth factor signaling; plays a role in regulation of neuronal cell proliferation [RGD, Feb 2006]

Uniprot Description

FGFR1 iso2: a receptor tyrosine kinase of the highly-conserved fibroblast growth factor receptor (FGFR). Binds both acidic and basic fibroblast growth factors and is involved in limb induction. Point mutations cause Pfeffer syndrome (finger and toe malformations and other skeletal errors) and dominant Kallmann syndrome 2. Stem cell leukemia lymphoma syndrome (SCLL) may be caused by a t(8;13)(p12;q12) translocation that fuses a zinc finger gene, ZNF198, to FGFR1. Various myeloproliferative disorders have been linked to translocations that fuse FGFR1 to FOP, FIM, CEP1 or the atypical kinase, Bcr. Inhibitor: SU5402. 20 isoforms of the human protein produced by alternative splicing have been described.

Protein type: EC 2.7.10.1; Oncoprotein; Kinase, protein; Protein kinase, tyrosine (receptor); Protein kinase, TK; Membrane protein, integral; TK group; FGFR family

Cellular Component: cytoplasmic membrane-bound vesicle; integral to membrane; plasma membrane; nucleus; cytosol; receptor complex

Molecular Function: heparin binding; identical protein binding; protein binding; protein homodimerization activity; fibroblast growth factor binding; fibroblast growth factor receptor activity; protein-tyrosine kinase activity; protein complex binding; cell adhesion molecule binding; glycoprotein binding; ATP binding

Biological Process: paraxial mesoderm development; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; cell maturation; middle ear morphogenesis; negative regulation of transcription from RNA polymerase II promoter; orbitofrontal cortex development; positive regulation of MAP kinase activity; sensory perception of sound; positive regulation of MAPKKK cascade; ureteric bud development; induction of an organ; salivary gland morphogenesis; positive regulation of cell proliferation; midbrain development; positive regulation of mesenchymal cell proliferation; chondrocyte differentiation; negative regulation of osteoblast differentiation; angiogenesis; neurite development; embryonic limb morphogenesis; central nervous system neuron development; positive regulation of cardiac muscle cell proliferation; inner ear morphogenesis; fibroblast growth factor receptor signaling pathway; motogenic signaling involved in postnatal olfactory bulb interneuron migration; in utero embryonic development; stem cell maintenance; outer ear morphogenesis; positive regulation of cell cycle; regulation of cell proliferation; neuroblast division in the ventricular zone; mesenchymal cell differentiation; generation of neurons; regulation of gene expression; blood vessel morphogenesis; auditory receptor cell development; positive regulation of transcription from RNA polymerase II promoter; regulation of sensory perception of pain; brain development; positive regulation of neuron differentiation; regulation of lateral mesodermal cell fate specification; lung development