Mouse Cyclin-E1 ELISA Kit | CCNE1 elisa kit

Mouse CCNE1 (Cyclin-E1) ELISA Kit

Cat. Num. AAA2533475

• Gene Names: CCNE1; CCNE; pCCNE1
• Reactivity: Mouse
• Synonyms: Cyclin-E1; Mouse CCNE1 (Cyclin-E1) ELISA Kit; CCNE1 elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes natural and recombinantMouseCCNE1. No significant cross-reactivity or interference between MouseCCNE1 and analogues was observed.
• Sequence Length: 410

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 78.125-5000pg/mL
• Sensitivity: Min: 46.875pg/mL; Max: 5000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCNE1 elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of MouseCCNE1concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CCNE1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CCNE1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CCNE1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CCNE1.You can calculate the concentration of CCNE1in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 23273784
• NCBI GeneID: 898
• Molecular Weight: 45,150 Da
• NCBI Official Full Name: Cyclin E1
• NCBI Official Synonym Full Names: cyclin E1
• NCBI Official Symbol: CCNE1
• NCBI Official Synonym Symbols: CCNE; pCCNE1
• NCBI Protein Information: G1/S-specific cyclin-E1
• UniProt Protein Name: G1/S-specific cyclin-E1
• UniProt Gene Name: CCNE1
• UniProt Synonym Gene Names: CCNE
• UniProt Entry Name: CCNE1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. [provided by RefSeq, Jul 2008]

Uniprot Description

CCNE1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Two alternatively spliced isoforms have been described.

Protein type: Cell cycle regulation; Nuclear receptor co-regulator; Activator

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: centrosome; cyclin-dependent protein kinase holoenzyme complex; cytosol; nucleoplasm; nucleus

Molecular Function: androgen receptor binding; cyclin-dependent protein kinase regulator activity; kinase activity; protein binding; protein complex binding; protein kinase binding; transcription coactivator activity

Biological Process: androgen receptor signaling pathway; antral ovarian follicle growth; cell division; cellular response to nutrient; DNA replication initiation; G1/S transition of mitotic cell cycle; G1/S-specific transcription in mitotic cell cycle; liver development; mitotic cell cycle; organ regeneration; positive regulation of cell differentiation; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; regulation of cell cycle; regulation of protein kinase activity; response to corticosterone stimulus; response to cytokine stimulus; response to drug; response to estradiol stimulus; response to ethanol; response to methylmercury; response to progesterone stimulus; response to purine; response to vitamin E; Wnt receptor signaling pathway