Rabbit anti-Human Cyclin E1 Polyclonal Antibody | anti-CCNE1 antibody

Anti-Cyclin E1 Antibody

Cat. Num. AAA417170

• Gene Names: CCNE1; CCNE
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Affinity purified
• Synonyms: Cyclin E1; Polyclonal Antibody; Anti-Cyclin E1 Antibody; Anti-Cyclin E1 (phospho-Thr395) Antibody; anti-CCNE1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Cyclin E1 (phospho-Thr395) Antibody detects endogenous levels of Cyclin E1only when phosphorylated at threonine395.
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 410

• Applicable Applications for anti-CCNE1 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50~100
• Storage Buffer: Rabbit IgG in phosphate buffered saline (without Mg< sup>2< /sup>+ and Ca< sup>2< /sup>+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Immunogen: The antibody was produced against synthesized phosphopeptide derived from human Cyclin E1 around the phosphorylation site of threonine395 (L-L-TP-P-P).
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 23273784
• NCBI GeneID: 898
• Molecular Weight: 45,150 Da
• NCBI Official Full Name: Cyclin E1
• NCBI Official Synonym Full Names: cyclin E1
• NCBI Official Symbol: CCNE1
• NCBI Official Synonym Symbols: CCNE
• NCBI Protein Information: G1/S-specific cyclin-E1; cyclin E variant ex5del; cyclin E variant ex7del; cyclin Es; cyclin Et
• UniProt Protein Name: G1/S-specific cyclin-E1
• UniProt Gene Name: CCNE1
• UniProt Entry Name: CCNE1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. [provided by RefSeq, Jul 2008]

Uniprot Description

CCNE1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Two alternatively spliced isoforms have been described.

Protein type: Nuclear receptor co-regulator; Activator; Cell cycle regulation

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: nucleoplasm; cyclin-dependent protein kinase holoenzyme complex; nucleus; cytosol

Molecular Function: protein binding; androgen receptor binding; transcription coactivator activity; kinase activity; cyclin-dependent protein kinase regulator activity; protein kinase binding

Biological Process: G1/S-specific transcription in mitotic cell cycle; Wnt receptor signaling pathway; DNA replication initiation; cell division; regulation of cell cycle; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; mitotic cell cycle; regulation of protein kinase activity; protein amino acid phosphorylation; G1/S transition of mitotic cell cycle