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Immunofluorescence (IF) (Immunofluorescence staining of P4HB in NIH-3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Mouse P4HB monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.)

Rabbit anti-Mouse P4HB Monoclonal Antibody | anti-P4HB antibody

Recombinant Anti-P4HB Antibody, Rabbit Monoclonal

Gene Names
P4hb; PDI; Thbp; ERp59; Pdia1
Reactivity
Mouse
Applications
Immunocytochemistry, Immunofluorescence
Purity
Protein A
Synonyms
P4HB; Monoclonal Antibody; Recombinant Anti-P4HB Antibody; Rabbit Monoclonal; P4HB Antibody; Rabbit MAb; prolyl 4-hydroxylase; beta polypeptide; Anti-ERp59 Antibody; Anti-PDI Antibody; Anti-Pdia1 Antibody; Anti-Thbp Antibody; anti-P4HB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
016
Specificity
Mouse P4HB
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-P4HB antibody
Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ICC/IF: 1:20-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse P4HB Protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse P4HB (rM P4HB).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence staining of P4HB in NIH-3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Mouse P4HB monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.)

Immunofluorescence (IF) (Immunofluorescence staining of P4HB in NIH-3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Mouse P4HB monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.)
Related Product Information for anti-P4HB antibody
Protein disulfide-isomerase, also known as Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55 and P4HB, is a peripheral membrane protein that belongs to the protein disulfide isomerase family. P4HB is highly abundant. In some cell types, it seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources. P4HB localizes near CD4-enriched regions on lymphoid cell surfaces. It is identified by mass spectrometry in melanosome fractions from stage I to stage IV. P4HB reduces and may activate fusogenic properties of HIV-1 gp12 surface protein, thereby enabling HIV-1 entry into the cell. P4HB catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, it seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. P4HB may therefore cause structural modifications of exofacial proteins. Inside the cell, it seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, P4HB functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). P4HB may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts as a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.
References
Kivirikko KI, et al., 1989, FASEB J., 3 (5): 1609-17. Pihlajaniemi T, et al., 1991, J Hepatol., 13, Suppl 3: S2 Fenouillet E., et al., 2001, J. Infect. Dis. 183:744-752. Gevaert K., et al., 2003, Nat. Biotechnol. 21:566-569. Barbouche R., et al., 2003, J. Biol. Chem. 278:3131-3136.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
57,058 Da
NCBI Official Full Name
protein disulfide-isomerase
NCBI Official Synonym Full Names
prolyl 4-hydroxylase, beta polypeptide
NCBI Official Symbol
P4hb
NCBI Official Synonym Symbols
PDI; Thbp; ERp59; Pdia1
NCBI Protein Information
protein disulfide-isomerase
UniProt Protein Name
Protein disulfide-isomerase
UniProt Gene Name
P4hb
UniProt Synonym Gene Names
Pdia1; PDI; ER protein 59; ERp59
UniProt Entry Name
PDIA1_MOUSE

Uniprot Description

PDIA1: This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Homodimer. Monomers and homotetramers may also occur. Also constitutes the structural subunit of prolyl 4-hydroxylase and of the microsomal triacylglycerol transfer protein MTTP in mammalian cells. Stabilizes both enzymes and retain them in the ER without contributing to the catalytic activity. Binds UBQLN1. Binds to CD4, and upon HIV-1 binding to the cell membrane, is part of a P4HB/PDI-CD4-CXCR4-gp120 complex. Belongs to the protein disulfide isomerase family.

Protein type: EC 5.3.4.1; Endoplasmic reticulum; Isomerase; Nuclear receptor co-regulator; Oxidoreductase

Cellular Component: endoplasmic reticulum; ER-Golgi intermediate compartment; external side of plasma membrane; extracellular matrix; focal adhesion

Molecular Function: enzyme binding; integrin binding; peptidyl-proline 4-dioxygenase activity; procollagen-proline 4-dioxygenase activity; protein binding; protein disulfide isomerase activity; protein heterodimerization activity

Biological Process: peptidyl-proline hydroxylation to 4-hydroxy-L-proline; positive regulation of virion penetration into host cell; protein folding

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Product Notes

The P4HB p4hb (Catalog #AAA8111852) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-P4HB Antibody, Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's P4HB can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:20-1:100. Researchers should empirically determine the suitability of the P4HB p4hb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "P4HB, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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