Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Human Cyclin Dependent Kinase 5 (CDK5) Polyclonal Antibody | anti-CDK5 antibody
Polyclonal Antibody to Cyclin Dependent Kinase 5 (CDK5)
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- DSCNGDLDP EIVKSFLFQL LKGLGFCHSR NVLHRDLKPQ NLLINRNGEL KLADFGLARA FGIPVRCYSA EVVTLWYRPP DVLFGAKLYS TSIDMWSAGC IFAELANAGR PLFPGNDVDD QLKRIFRLLG TPTEEQWPSM TKLPDYKPYP MYPATTSLVN VVPKLNATGR DLLQNLLKCN PVQ
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Brain tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Brain tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the protein encoded by this gene does not directly control cell cycle regulation. Instead the protein, which is predominantly expressed at high levels in mammalian postmitotic central nervous system neurons, functions in diverse processes such as synaptic plasticity and neuronal migration through phosphorylation of proteins required for cytoskeletal organization, endocytosis and exocytosis, and apoptosis. In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]
Uniprot Description
CDK5: a protein kinase of the CDK family. Unlike other members of this family, it is not activated by cyclins but by p35 (CDK5R1) and p39. An important regulator of neuronal positioning during brain development. May also play a role in synaptogenesis and neurotransmission. Substrates include TAU, MAP2, NF-H and -M, Nudel, PDE6, beta-catenin, amphyphysin, dynamin I, synapsin 1, Munc-18, and NMDA receptor 2A. Plays a role in myogenesis, haematopoietic cell differentiation, spermatogenesis, insulin secretion, and lens differentiation. Implicated in the pathology of neurofibrillary tangles and formation of senile plaques, hallmarks of Alzheimer?s disease. Induces tau phosphorylation and aggregation and neurofibrillary tangle deposition and neurodegeneration in in vitro and in vivo animal models. Brain samples from Alzeimer?s pateints show elevated CDK5 activity.
Protein type: CDK family; CDK/CDK5 subfamily; CDK5 subfamily; CMGC group; Cell cycle regulation; EC 2.7.11.1; Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 7q36.1
Cellular Component: axon; cell junction; cell soma; cyclin-dependent protein kinase 5 activator complex; cytoplasm; cytosol; dendrite; filopodium; growth cone; lamellipodium; membrane; microtubule; neuromuscular junction; nucleoplasm; nucleus; perikaryon; plasma membrane; postsynaptic density; postsynaptic membrane; presynapse
Molecular Function: acetylcholine receptor activator activity; ATP binding; cyclin-dependent protein kinase activity; ephrin receptor binding; ErbB-2 class receptor binding; ErbB-3 class receptor binding; kinase activity; microtubule binding; p53 binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; tau-protein kinase activity
Biological Process: axon extension; behavioral response to cocaine; cell cycle; cell division; cell proliferation; cell-matrix adhesion; cellular response to amyloid-beta; central nervous system neuron development; cerebellar cortex formation; corpus callosum development; cortical actin cytoskeleton organization and biogenesis; dendrite morphogenesis; excitatory postsynaptic potential; hippocampus development; intracellular protein transport; layer formation in cerebral cortex; microtubule cytoskeleton organization; motor axon guidance; negative regulation of axon extension; negative regulation of cell cycle; negative regulation of neuron death; negative regulation of protein export from nucleus; negative regulation of protein ubiquitination; negative regulation of proteolysis; negative regulation of synaptic plasticity; negative regulation of transcription, DNA-dependent; neuron apoptosis; neuron differentiation; neuron migration; neuron projection development; nucleocytoplasmic transport; oligodendrocyte differentiation; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphorylation; positive regulation of actin cytoskeleton reorganization; positive regulation of calcium ion-dependent exocytosis; positive regulation of neuron apoptotic process; positive regulation of protein binding; positive regulation of protein kinase activity; positive regulation of protein targeting to membrane; positive regulation of receptor activity; protein amino acid phosphorylation; protein autophosphorylation; receptor catabolic process; receptor clustering; regulation of apoptosis; regulation of cell cycle arrest; regulation of cell migration; regulation of macroautophagy; regulation of synaptic plasticity; regulation of synaptic transmission, glutamatergic; response to wounding; rhythmic process; Schwann cell development; sensory perception of pain; serine phosphorylation of STAT protein; skeletal muscle tissue development; synaptic transmission; synaptic transmission, dopaminergic; synaptic transmission, glutamatergic; synaptic vesicle endocytosis; synaptic vesicle exocytosis; synaptogenesis; visual learning
Disease: Lissencephaly 7 With Cerebellar Hypoplasia
Research Articles on CDK5
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Product Notes
The CDK5 cdk5 (Catalog #AAA2003098) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Cyclin Dependent Kinase 5 (CDK5) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin Dependent Kinase 5 (CDK5) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CDK5 cdk5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- DSCNGDLDP EIVKSFLFQL LKGLGFCHSR NVLHRDLKPQ NLLINRNGEL KLADFGLARA FGIPVRCYSA EVVTLWYRPP DVLFGAKLYS TSIDMWSAGC IFAELANAGR PLFPGNDVDD QLKRIFRLLG TPTEEQWPSM TKLPDYKPYP MYPATTSLVN VVPKLNATGR DLLQNLLKCN PVQ. It is sometimes possible for the material contained within the vial of "Cyclin Dependent Kinase 5 (CDK5), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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