Rabbit anti-Human Langerin/CD207 Antibody | anti-CD207 antibody

Langerin/CD207 (Marker of Langerhans Cells)

Cat. Num. AAA4381143

• Gene Names: CD207; CLEC4K
• Reactivity: Human
• Applications: ELISA
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Langerin/CD207; Langerin/CD207 (Marker of Langerhans Cells); C-type lectin domain family 4 member K; CD207; CLEC4K; Langerhans cell specific c type lectin; Langerin; anti-CD207 antibody

• Host: Rabbit
• Reactivity: Human
• Isotype: IgG
• Clone Number: LGRN/3136R
• Specificity: Langerhans cells (LCs) are a subset of immature dendritic cells (DCs) that specifically localize in the epidermis and other mucosal epithelia. Epidermal LCs possess strong immuno-stimulatory capacity and play a central role in the initiation and regulation of immune responses. Langerin (CD207) is a Ca2+-dependent, C-type lectin domain containing, type II transmembrane protein that induces epidermal LCs to differentiate into Birbeck granules (BG). BGs are organelles with superimposing and zippering membranes that influence proper class I type antigen presentation to the circulating T cells. Human spleen, lymph node, thymus, liver, lung and heart express Langerin protein.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 328

• Applicable Applications for anti-CD207 antibody: ELISA (EIA)
• Application Notes: ELISA: Use Ab at 2-4ug/ml for coating (Order Ab without BSA)
• Positive Control: HeLa or A549 cells. Skin.
• Immunogen: Recombinant fragment (around aa74-213) of human Langerin protein (exact sequence is proprietary)
• Cellular Localization: Cell Surface & Cytoplasmic
• Hu-chromosome Location: 2p13.3
• Antibody Type: Recombinant Rabbit Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-Page

(SDS-PAGE Analysis Purified Langerin Recombinant Rabbit Monoclonal Antibody (LGRN/3136R). Confirmation of Integrity and Purity of Antibody.)

References

Valladeau, J., et al. 2000. Langerin, a novel C-type lectin specific to Langerhans cells, is an endocytic receptor that induces the formation of Birbeck granules. Immunity 12: 71-81.

NCBI and Uniprot Product Information

• NCBI GI #: 557357745
• NCBI GeneID: 50489
• NCBI Accession #: NP_056532.4
• NCBI GenBank Nucleotide #: NP_056532.4
• UniProt Accession #: Q9UJ71
• Molecular Weight: 48kDa
• NCBI Official Full Name: C-type lectin domain family 4 member K
• NCBI Official Synonym Full Names: CD207 molecule
• NCBI Official Symbol: CD207
• NCBI Official Synonym Symbols: CLEC4K
• NCBI Protein Information: C-type lectin domain family 4 member K
• UniProt Protein Name: C-type lectin domain family 4 member K
• Protein Family: C-type lectin domain family
• UniProt Gene Name: CD207
• UniProt Synonym Gene Names: CLEC4K
• UniProt Entry Name: CLC4K_HUMAN

NCBI Description

The protein encoded by this gene is expressed only in Langerhans cells which are immature dendritic cells of the epidermis and mucosa. It is localized in the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed and zippered membranes. It is a C-type lectin with mannose binding specificity, and it has been proposed that mannose binding by this protein leads to internalization of antigen into Birbeck granules and providing access to a nonclassical antigen-processing pathway. Mutations in this gene result in Birbeck granules deficiency or loss of sugar binding activity. [provided by RefSeq, Aug 2010]

Uniprot Description

langerin: Calcium-dependent lectin displaying mannose-binding specificity. Induces the formation of Birbeck granules (BGs); is a potent regulator of membrane superimposition and zippering. Binds to sulfated as well as mannosylated glycans, keratan sulfate (KS) and beta-glucans. Facilitates uptake of antigens and is involved in the routing and/or processing of antigen for presentation to T cells. Major receptor on primary Langerhans cells for Candida species, Saccharomyces species, and Malassezia furfur. Protects against human immunodeficiency virus-1 (HIV-1) infection. Binds to high-mannose structures present on the envelope glycoprotein which is followed by subsequent targeting of the virus to the Birbeck granules leading to its rapid degradation. Homotrimer. Exclusively expressed by Langerhans cells. Expressed in astrocytoma and malignant ependymoma, but not in normal brain tissues.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: early endosome membrane; endocytic vesicle; plasma membrane

Molecular Function: carbohydrate binding; mannose binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; defense response to virus; receptor-mediated endocytosis

Disease: Birbeck Granule Deficiency