Western Blot (WB)
(Anti- Cyclin D1 antibody, MBS177038, All Western blottingAll lanes: Anti-CCND1(MBS177038) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 40ugLane 2: Human Placenta Tissue Lysate at 40ugLane 3: Rat Brain Tissue Lysate at 40ugLane 4: MCF-7 Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugLane 6: SW620 Whole Cell Lysate at 40ugLane 7: MM231 Whole Cell Lysate at 40ugPredicted bind size: 33KDObserved bind size: 33KD )
Rabbit anti-Human Cyclin D1 Polyclonal Antibody | anti-CCND1 antibody
Anti- Cyclin D1 antibody
Western Blot (WB)
(Anti- Cyclin D1 antibody, MBS177038, All Western blottingAll lanes: Anti-CCND1(MBS177038) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 40ugLane 2: Human Placenta Tissue Lysate at 40ugLane 3: Rat Brain Tissue Lysate at 40ugLane 4: MCF-7 Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugLane 6: SW620 Whole Cell Lysate at 40ugLane 7: MM231 Whole Cell Lysate at 40ugPredicted bind size: 33KDObserved bind size: 33KD )
Western Blot (WB)
(Anti- Cyclin D1 antibody, MBS177038, All Western blottingAll lanes: Anti-CCND1(MBS177038) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 40ugLane 2: Human Placenta Tissue Lysate at 40ugLane 3: Rat Brain Tissue Lysate at 40ugLane 4: MCF-7 Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugLane 6: SW620 Whole Cell Lysate at 40ugLane 7: MM231 Whole Cell Lysate at 40ugPredicted bind size: 33KDObserved bind size: 33KD )
Background: Cyclin D1, also known as CCND1, is a human gene. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclin D1 encodes the regulatory subunit of a holoenzyme that phosphorylates and inactivates the retinoblastoma protein and promotes progression through the G1-S phase of the cell cycle. Amplification or overexpression of cyclin D1 plays pivotal roles in the development of a subset of human cancers including parathyroid adenoma, breast cancer, colon cancer, lymphoma, melanoma, and prostate cancer. The cyclin D1 gene is overexpressed in human breast cancers and is required for oncogene-induced tumorigenesis. Brisken et al. (2003) found that prolactin (PRL; 176760) induced IGF2 (147470) mRNA and IGF2 induced cyclin D1 protein expression in mouse mammary epithelial cultures. And they also concluded that IGF2 is a mediator of prolactin-induced alveologenesis and that prolactin, IGF2, and cyclin D1 are components of a developmental pathway in mammary gland.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008]
Uniprot Description
CCND1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb.
Protein type: Activator; Oncoprotein; Cell cycle regulation; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: nucleoplasm; tight junction; membrane; transcriptional repressor complex; cyclin-dependent protein kinase holoenzyme complex; intracellular; cytosol; nucleus
Molecular Function: protein binding; enzyme binding; histone deacetylase binding; protein complex binding; cyclin-dependent protein kinase regulator activity; transcription corepressor activity; transcription factor binding; protein kinase binding; protein kinase activity
Biological Process: G1 DNA damage checkpoint; fat cell differentiation; lactation; establishment and/or maintenance of chromatin architecture; negative regulation of Wnt receptor signaling pathway; positive regulation of cyclin-dependent protein kinase activity; re-entry into mitotic cell cycle; positive regulation of mammary gland epithelial cell proliferation; negative regulation of epithelial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; protein amino acid phosphorylation; response to vitamin E; response to magnesium ion; Leydig cell differentiation; response to iron ion; response to corticosterone stimulus; response to X-ray; response to drug; organ regeneration; Notch signaling pathway; transcription, DNA-dependent; unfolded protein response; mammary gland epithelial cell proliferation; response to organic nitrogen; liver development; response to ethanol; response to estrogen stimulus; cell division; mitotic cell cycle; positive regulation of protein amino acid phosphorylation; response to calcium ion; response to DNA damage stimulus; G1/S transition of mitotic cell cycle
Disease: Von Hippel-lindau Syndrome; Myeloma, Multiple
Research Articles on CCND1
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Product Notes
The CCND1 ccnd1 (Catalog #AAA177038) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti- Cyclin D1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin D1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the CCND1 ccnd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclin D1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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