Immunohistochemistry (IHC)
(Immunochemical staining of human CCNB2 in human adrenal gland with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Rabbit Cyclin B2 Polyclonal Antibody | anti-CCNB2 antibody
Anti-Cyclin B2 Antibody, Rabbit Polyclonal
Predicted to React with: Cynomolgus (Species predicted to react based on 100% sequence homology)
Predicted to React with: Cynomolgus (Species predicted to react based on 100% sequence homology)
ICC/IF: 1:300-1:10,000
Immunohistochemistry (IHC)
(Immunochemical staining of human CCNB2 in human adrenal gland with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human CCNB2 in human adrenal gland with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunofluorescence (IF)
(Immunofluorescence staining of CCNB2 in SW480 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CCNB2 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of CCNB2 in SW480 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CCNB2 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.)
NCBI and Uniprot Product Information
NCBI Description
Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control. [provided by RefSeq, Jul 2008]
Uniprot Description
CCNB2: Essential for the control of the cell cycle at the G2/M (mitosis) transition. Belongs to the cyclin family. Cyclin AB subfamily.
Protein type: Cell cycle regulation
Chromosomal Location of Human Ortholog: 15q22.2
Cellular Component: microtubule cytoskeleton; nucleoplasm; centrosome; membrane; cytosol
Molecular Function: protein binding; protein kinase binding
Biological Process: mitosis; thymus development; cell division; regulation of cell cycle; in utero embryonic development; mitotic nuclear envelope disassembly; T cell homeostasis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; growth
Research Articles on CCNB2
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Product Notes
The CCNB2 ccnb2 (Catalog #AAA8101324) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cyclin B2 Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Cynomolgus (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin B2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF). IHC-P: 1:500-1:2000 ICC/IF: 1:300-1:10,000. Researchers should empirically determine the suitability of the CCNB2 ccnb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclin B2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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