Western Blot (WB)
(Western blot analysis of Cyclin B1 expression in Hela (A) whole cell lysates.)
Rabbit Cyclin B1 Polyclonal Antibody | anti-CCNB1 antibody
Anti-Cyclin B1 Antibody
Western Blot (WB)
(Western blot analysis of Cyclin B1 expression in Hela (A) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Cyclin B1 expression in Hela (A) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Cyclin B1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Cyclin B1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]
Uniprot Description
CCNB1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Complexes with cdc2 to form the maturation-promoting factor (MPF). Two alternatively spliced isoforms have been found, a constitutively expressed form and a cell cycle-regulated form that is expressed predominantly during G2/M phase.
Protein type: Activator; Cell cycle regulation
Chromosomal Location of Human Ortholog: 5q12
Cellular Component: centrosome; cytoplasm; cytosol; membrane; nucleoplasm; nucleus; spindle pole
Molecular Function: histone kinase activity; patched binding; protein binding; protein kinase binding
Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; G1/S transition of mitotic cell cycle; G2/M transition of mitotic cell cycle; gut development; in utero embryonic development; mitotic cell cycle; mitotic metaphase plate congression; mitotic nuclear envelope disassembly; mitotic spindle stabilization; negative regulation of protein amino acid phosphorylation; oocyte maturation; positive regulation of attachment of spindle microtubules to kinetochore; positive regulation of cardiac muscle cell proliferation; positive regulation of fibroblast proliferation; positive regulation of histone phosphorylation; positive regulation of mitotic cell cycle; positive regulation of mRNA 3'-end processing; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein complex assembly; regulation of cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; response to DDT; response to drug; response to mechanical stimulus; spermatogenesis; tissue regeneration; ventricular cardiac muscle cell development
Research Articles on CCNB1
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Product Notes
The CCNB1 ccnb1 (Catalog #AAA8242036) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cyclin B1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin B1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/1000 - 1/2000); Immunohistochemistry: (1/200 - 1/500). Researchers should empirically determine the suitability of the CCNB1 ccnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclin B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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