Cytochrome P450 7A1 (CYP7A1) Recombinant Protein | CYP7A1 recombinant protein

Recombinant Cytochrome P450 7A1 (CYP7A1)

Cat. Num. AAA2018469

• Reactivity: Organism: Mus musculus (Mouse)
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cytochrome P450 7A1 (CYP7A1); Recombinant Cytochrome P450 7A1 (CYP7A1); CYP7A1 recombinant protein

• Host: E Coli
• Reactivity: Organism: Mus musculus (Mouse)
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LFKT AHKARERLAE SLKHKNLYMR DQVSELIRLR MFLNDTLSTF DDMEKAKTHL VILWASQANT IPATFWSLFQ MIRSPEAMKA ASEEVNGALQ SAGQELSSGG NAIYLDQEQL NNLPVLDSII KEALRLSSAS LNIRTAKEDF TLHLEDGSYN IRKDDIIALY PQLMHLDPEI YPDPLTFKYD RYLDESGKAKTTFYRNGNKL KYFYMPFGSG ATICPGRLFA VQEIKQFLIL MLSYFEL
• Sequence Length: 503

• Applicable Applications for CYP7A1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Expression System: Prokaryotic expression
• Residues: Leu227~Leu467 (Accession # Q64505) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane
• Endotoxin Level: less than 1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.9
• Reconstitution: Reconstitute in ddH2O
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 31542445
• NCBI GeneID: 13122
• NCBI Accession #: NP_031850.2
• NCBI GenBank Nucleotide #: NM_007824.2
• UniProt Accession #: Q64505; Q8BFR7
• Molecular Weight: 31.5kDa
• NCBI Official Full Name: cholesterol 7-alpha-monooxygenase
• NCBI Official Synonym Full Names: cytochrome P450, family 7, subfamily a, polypeptide 1
• NCBI Official Symbol: Cyp7a1
• NCBI Protein Information: cholesterol 7-alpha-monooxygenase; CYPVII; cytochrome P450 7A1; cytochrome P450, 7a1; cholesterol 7 alpha hydroxylase; cholesterol 7-alpha-hydroxylase
• UniProt Protein Name: Cholesterol 7-alpha-monooxygenase
• Protein Family: Cholesterol 7-alpha-monooxygenase
• UniProt Gene Name: Cyp7a1
• UniProt Synonym Gene Names: Cyp7
• UniProt Entry Name: CP7A1_MOUSE

Uniprot Description

CYP7A1: Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis. Belongs to the cytochrome P450 family.

Protein type: EC 1.14.13.17; Lipid Metabolism - primary bile acid biosynthesis; Endoplasmic reticulum; Oxidoreductase

Cellular Component: intracellular membrane-bound organelle; membrane; endoplasmic reticulum

Molecular Function: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; cholesterol 7-alpha-monooxygenase activity; metal ion binding; iron ion binding; heme binding; oxidoreductase activity; monooxygenase activity

Biological Process: steroid metabolic process; cholesterol metabolic process; bile acid biosynthetic process; cholesterol homeostasis; lipid metabolic process; cholesterol catabolic process